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Literature summary for 3.5.3.1 extracted from

  • Siddappa, S.; Basrur, V.; Ravishankar Rai, V.; Marathe, G.K.
    Biochemical and functional characterization of an atypical plant L-arginase from Cilantro (Coriandrum sativam L.) (2018), Int. J. Biol. Macromol., 118, 844-856 .
    View publication on PubMed
Search for more literature for 3.5.3.1

Activating Compound

Activating Compound Comment Organism Structure
putrescine at 0.1 mM concentration enhances arginase activity by 18% Coriandrum sativum
spermidine slowly enhances activity between 5 mM and 25 mM Coriandrum sativum
spermine 1 mM, enhances arginase activity by 33% Coriandrum sativum

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol
-
 Coriandrum sativum
dithiothreitol
-
 Coriandrum sativum
EDTA
-
 Coriandrum sativum
L-argininamide mixed type of inhibition Coriandrum sativum
L-canavanine non-competitive type of inhibition Coriandrum sativum
L-homoarginine mixed type of inhibition Coriandrum sativum
N-omega-hydroxy-L-arginine moderate inhibition Coriandrum sativum
spermidine inhibition in a dose-dependent fashion below 5 mM and above 25 mM Coriandrum sativum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
29.5
-
 L-arginine pH 7.8, 37°C Coriandrum sativum

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 0.5 mM, 43% of the activity compared to activity with Mn2+ Coriandrum sativum
Mn2+ maximum activity (100%) with 1 mM Mn2+. 0.5mM of Mn2+ exhibits 70% of total activity Coriandrum sativum
Ni2+ 1 mM, 15% of the activity compared to activity with Mn2+ Coriandrum sativum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
160000
-
 gel filtration Coriandrum sativum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-arginine + H2O Coriandrum sativum
-
 L-ornithine + urea
-
 ?

Organism

Organism UniProt Comment Textmining
Coriandrum sativum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Coriandrum sativum

Source Tissue

Source Tissue Comment Organism Textmining
seed
-
 Coriandrum sativum
-
seedling
-
 Coriandrum sativum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
81.728
-
 pH 7.8, 37°C Coriandrum sativum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-arginine + H2O
-
 Coriandrum sativum L-ornithine + urea
-
 ?
L-arginine + H2O the enzyme does not hydrolyse D-arginine and other arginine analogues (L-agmatine, L-canavanine, L-argininamide and L-homoarginine) Coriandrum sativum L-ornithine + urea
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40 60
-
 Coriandrum sativum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8.1
-
 Coriandrum sativum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
10.96
-
 L-canavanine pH 7.8, 37°C Coriandrum sativum
179.3
-
 N-omega-hydroxy-L-arginine pH 7.8, 37°C Coriandrum sativum

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.33
-
 pH 7.8, 37°C Coriandrum sativum EDTA
1.196
-
 pH 7.8, 37°C Coriandrum sativum 2-mercaptoethanol
7.789
-
 pH 7.8, 37°C Coriandrum sativum dithiothreitol

Expression

Organism Comment Expression
Coriandrum sativum increase in activity to both biotic and abiotic stress up

General Information

General Information Comment Organism
metabolism arginase is one of the key enzymes responsible for maintaining the essential levels of nitrogen among plants Coriandrum sativum