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Literature summary for 3.5.3.1 extracted from
- Purification and characterization of buffalo liver L-arginase and its kinetic properties with dihydropyrimidine and metal ions (2016), Indian J. Exp. Biol., 54, 414-419 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 1-amino-6-methyl-4-phenyl-2-thioxo-1,2,3,4-tetrahydropyrimidine-5-carboxylic acid methyl ester | activity is enhanced in the presence of the dihydropyrimidine derivative | Bison bison |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 7.14 | - |
L-arginine | in absence of dihydropyrimidine derivative, pH and temperature not specified in the publication | Bison bison | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 60% of the activation as compared to Mn2+ | Bison bison | |
| Mg2+ | 90% of the activation as compared to Mn2+ | Bison bison | |
| Mn2+ | acts as co-factor and as activator | Bison bison | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 47000 | - |
SDS-PAGE | Bison bison |
| 118000 | - |
gel filtration | Bison bison |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-arginine + H2O | Bison bison | - |
L-ornithine + urea | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bison bison | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 2857 purification fold and a 20% recovery | Bison bison |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Bison bison | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 11.5 | - |
pH and temperature not specified in the publication | Bison bison |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-arginine + H2O | - |
Bison bison | L-ornithine + urea | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 47000, SDS-PAGE | Bison bison |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Bison bison |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.5 | - |
in absence and presence of dihydropyrimidine derivative | Bison bison |
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