Literature summary for 3.5.3.1 extracted from

da Silva, E.R.; da Silva, M.F.; Fischer, H.; Mortara, R.A.; Mayer, M.G.; Framesqui, K.; Silber, A.M.; Floeter-Winter, L.M.
Biochemical and biophysical properties of a highly active recombinant arginase from Leishmania (Leishmania) amazonensis and subcellular localization of native enzyme (2008), Mol. Biochem. Parasitol., 159, 104-111.

Search for more literature for 3.5.3.1

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21 cells	Leishmania amazonensis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
21.5	-	L-arginine	native enzyme	Leishmania amazonensis
23.9	-	L-arginine	native enzyme	Leishmania amazonensis

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
glycosome	-	Leishmania amazonensis	20015	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mn2+	a manganese wash at room temperature is the best condition to purify active enzyme, activity in the presence of 10 mM Mn2+ is at least 15fold higher than in the absence of Mn2+	Leishmania amazonensis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-arginine + H2O	Leishmania amazonensis	-	L-ornithine + urea	-	?

Organism

Organism	UniProt	Comment	Textmining
Leishmania amazonensis	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
HiTrap Ni2+-chelating Sepharose column chromatography	Leishmania amazonensis

Source Tissue

Source Tissue	Comment	Organism	Textmining
promastigote	-	Leishmania amazonensis	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
33	-	homogenate supernatant, in the presence of 10 mM Mn2+	Leishmania amazonensis
1140	-	35fold purified recombinant enzyme, in the presence of 10 mM Mn2+	Leishmania amazonensis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-arginine + H2O	-	Leishmania amazonensis	L-ornithine + urea	-	?

Subunits

Subunits	Comment	Organism
trimer	the trimeric state is the active form of the protein, X-ray scattering	Leishmania amazonensis

Synonyms

Synonyms	Comment	Organism
L-arginine amidinohydrolase	-	Leishmania amazonensis

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)