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Literature summary for 3.5.3.1 extracted from
- Purification and characterization of Helicobacter pylori arginase, RocF: unique features among the arginase superfamily (2004), Eur. J. Biochem., 271, 1952-1962.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | activation at 50-55°C in presence of Co2+ | Helicobacter pylori |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene rocF, functional expression in Escherichia coli DH5alpha as His-tagged enzyme | Helicobacter pylori |
General Stability
| General Stability | Organism |
|---|---|
| dialysis results in in loss of over 90% activity, highly unstable enzyme | Helicobacter pylori |
| recombinant protein, highly unstable | Helicobacter pylori |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | 50% inhibition at 0.8 mM | Helicobacter pylori |  |
| dithiothreitol | 50% inhibition at 0.025 mM | Helicobacter pylori | |
| DTT | 50% inhibition at 0.025 mM | Helicobacter pylori | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 21.8 | - |
L-arginine | pH 7.1, 37°C | Helicobacter pylori | |
| 21.8 | - |
L-arginine | pH 7.1, 37°C, recombinant enzyme | Helicobacter pylori | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | required | Helicobacter pylori | |
| Co2+ | required, activates, best metal cofactor | Helicobacter pylori | |
| Mn2+ | activates slightly, 14.6% of the activity with Co2+ | Helicobacter pylori | |
| Mn2+ | 15-20% of the activity with Co2+ | Helicobacter pylori | |
| additional information | metalloenzyme, a metal ion is absolutely required for activity, no activation with Zn2+, Cu2+, Fe2+, Ca2+, and Mg2+ | Helicobacter pylori | |
| Ni2+ | activates slightly, 6.1% of the activity with Co2+ | Helicobacter pylori | |
| Ni2+ | 6-30% of the activity with Co2+ | Helicobacter pylori | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
x * 40000, SDS-PAGE, recombinant enzyme with His-tag | Helicobacter pylori |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-arginine + H2O | Helicobacter pylori | enzyme is involved in acid resistance and inhibits host nitric oxide production | L-ornithine + urea | - |
? | |
| L-arginine + H2O | Helicobacter pylori ATCC 43504 | enzyme is involved in acid resistance and inhibits host nitric oxide production | L-ornithine + urea | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Helicobacter pylori | - |
- |
- |
| Helicobacter pylori | - |
gene rocF | - |
| Helicobacter pylori ATCC 43504 | - |
gene rocF | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Escherichia coli | Helicobacter pylori |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, His-tagged recombinant protein, 1:2 dilution with 100% glycerol, 4 months, 45-65% loss of activity | Helicobacter pylori |
| -20°C, purified recombinant enzyme, 1:2 with glycerol, loss of 45-65% activity after 4 months, and of over 90% after 6 months | Helicobacter pylori |
| 4°C, His-tagged recombinant protein, 1:2 dilution with 100% glycerol, 1 week, 90% loss of activity | Helicobacter pylori |
| 4°C, purified recombinant enzyme, 1:2 with glycerol, over 90% loss of activity within 1 week | Helicobacter pylori |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-arginine + H2O | - |
Helicobacter pylori | L-ornithine + urea | - |
? | |
| L-arginine + H2O | enzyme is involved in acid resistance and inhibits host nitric oxide production | Helicobacter pylori | L-ornithine + urea | - |
? | |
| L-arginine + H2O | - |
Helicobacter pylori ATCC 43504 | L-ornithine + urea | - |
? | |
| L-arginine + H2O | enzyme is involved in acid resistance and inhibits host nitric oxide production | Helicobacter pylori ATCC 43504 | L-ornithine + urea | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 37800-40000, about, His-tagged enzyme, sequence calculation and SDS-PAGE | Helicobacter pylori |
| ? | x * 40000, SDS-PAGE, recombinant enzyme with His-tag | Helicobacter pylori |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | enzyme belongs to the arginase superfamily | Helicobacter pylori |
| RocF | - |
Helicobacter pylori |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
assay requires heat activation of enzyme in presence of metal cofactor at 50-55°C | Helicobacter pylori |
| 30 | - |
- |
Helicobacter pylori |
| 37 | - |
assay at | Helicobacter pylori |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 42 | no reproducible temperature preference within this range | Helicobacter pylori |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 55 | activation in presence of Co2+ | Helicobacter pylori |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.1 | - |
- |
Helicobacter pylori |
| 6.1 | - |
recombinant enzyme | Helicobacter pylori |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.3 | 8.7 | about 50% of amximal activity at pH 5.3 and pH 8.7 | Helicobacter pylori |
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