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Literature summary for 3.5.2.9 extracted from

  • Oke, M.; Oni, O.; Bello, R.; Samuel-Omoyajowo, K.; Senbadejo, T.
    Structure-function relationships of the 5-oxoprolinase subunit A guiding biological sciences students down the path less traveled (2019), Biochem. Mol. Biol. Educ., 47, 620-631 .
    View publication on PubMed
Search for more literature for 3.5.2.9

Cloned(Commentary)

Cloned (Comment) Organism
gene pxpA3, phylogenetic analysis Pseudomonas aeruginosa

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structure analysis, PDB ID 2XU2, and structure comparisons, structure-function relationships of 5-oxoprolinase subunit A, overview Pseudomonas aeruginosa

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 5-oxo-L-proline + 2 H2O Pseudomonas aeruginosa
-
 ADP + phosphate + L-glutamate
-
 ?
ATP + 5-oxo-L-proline + 2 H2O Pseudomonas aeruginosa ATCC 15692
-
 ADP + phosphate + L-glutamate
-
 ?
ATP + 5-oxo-L-proline + 2 H2O Pseudomonas aeruginosa 1C
-
 ADP + phosphate + L-glutamate
-
 ?
ATP + 5-oxo-L-proline + 2 H2O Pseudomonas aeruginosa PRS 101
-
 ADP + phosphate + L-glutamate
-
 ?
ATP + 5-oxo-L-proline + 2 H2O Pseudomonas aeruginosa DSM 22644
-
 ADP + phosphate + L-glutamate
-
 ?
ATP + 5-oxo-L-proline + 2 H2O Pseudomonas aeruginosa CIP 104116
-
 ADP + phosphate + L-glutamate
-
 ?
ATP + 5-oxo-L-proline + 2 H2O Pseudomonas aeruginosa LMG 12228
-
 ADP + phosphate + L-glutamate
-
 ?
ATP + 5-oxo-L-proline + 2 H2O Pseudomonas aeruginosa JCM 14847
-
 ADP + phosphate + L-glutamate
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa Q9HVR0 encoding subunit A
-
Pseudomonas aeruginosa 1C Q9HVR0 encoding subunit A
-
Pseudomonas aeruginosa ATCC 15692 Q9HVR0 encoding subunit A
-
Pseudomonas aeruginosa CIP 104116 Q9HVR0 encoding subunit A
-
Pseudomonas aeruginosa DSM 22644 Q9HVR0 encoding subunit A
-
Pseudomonas aeruginosa JCM 14847 Q9HVR0 encoding subunit A
-
Pseudomonas aeruginosa LMG 12228 Q9HVR0 encoding subunit A
-
Pseudomonas aeruginosa PRS 101 Q9HVR0 encoding subunit A
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 5-oxo-L-proline + 2 H2O
-
 Pseudomonas aeruginosa ADP + phosphate + L-glutamate
-
 ?
ATP + 5-oxo-L-proline + 2 H2O
-
 Pseudomonas aeruginosa ATCC 15692 ADP + phosphate + L-glutamate
-
 ?
ATP + 5-oxo-L-proline + 2 H2O
-
 Pseudomonas aeruginosa 1C ADP + phosphate + L-glutamate
-
 ?
ATP + 5-oxo-L-proline + 2 H2O
-
 Pseudomonas aeruginosa PRS 101 ADP + phosphate + L-glutamate
-
 ?
ATP + 5-oxo-L-proline + 2 H2O
-
 Pseudomonas aeruginosa DSM 22644 ADP + phosphate + L-glutamate
-
 ?
ATP + 5-oxo-L-proline + 2 H2O
-
 Pseudomonas aeruginosa CIP 104116 ADP + phosphate + L-glutamate
-
 ?
ATP + 5-oxo-L-proline + 2 H2O
-
 Pseudomonas aeruginosa LMG 12228 ADP + phosphate + L-glutamate
-
 ?
ATP + 5-oxo-L-proline + 2 H2O
-
 Pseudomonas aeruginosa JCM 14847 ADP + phosphate + L-glutamate
-
 ?

Subunits

Subunits Comment Organism
More structural bioinformatics analysis of four putative PxpA structures reveal that PxpA adopts a non-canonical TIM barrel fold with well-characterized TIM barrel enzyme features. Structure-function relationships of 5-oxoprolinase subunit A. Four putative PxpA three-dimensional structures are determined by structural genomics initiatives Pseudomonas aeruginosa

Synonyms

Synonyms Comment Organism
5-oxoprolinase
-
 Pseudomonas aeruginosa
PA4511
-
 Pseudomonas aeruginosa
PxpA prokaryotic 5-oxoprolinase subunit A Pseudomonas aeruginosa
pxpA3
-
 Pseudomonas aeruginosa

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Pseudomonas aeruginosa

General Information

General Information Comment Organism
evolution phylogenetic analysis suggests a relationship between taxonomic grouping and PxpA oligomerization Pseudomonas aeruginosa
additional information subunit A, PxpA, associates with the subunit B and C complex, PxpBC, to form a functional 5-oxoprolinase enzyme for conversion of 5-oxoproline to L-glutamate. PxpBC catalyses the first step of the reaction, which is phosphorylation of 5-oxoproline. PxpA is involved in the last two steps of the reaction: decyclization of the labile phosphorylated 5-oxoproline to the equally labile gamma-glutamylphosphate, and subsequent dephosphorylation to L-glutamate. Structural bioinformatics analysis of four putative PxpA structures reveal that PxpA adopts a non-canonical TIM barrel fold with well-characterized TIM barrel enzyme features. Structure-function relationships of 5-oxoprolinase subunit A, overview. PxpA forms a tunnel upon ligand binding, thus suggesting that the PxpABC complex employs the mechanism of substrate channeling to protect labile intermediates. Active site structure of PxpA Pseudomonas aeruginosa