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Literature summary for 3.5.2.9 extracted from
- The amino acid sequence of rat kidney 5-oxo-L-prolinase determined by cDNA cloning (1996), J. Biol. Chem., 271, 32293-32300.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| amino acid sequence determined by cDNA cloning, gene expressed in Escherichia coli | Rattus norvegicus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
component A 1 * 65500 + 1 * 50000, component B 1 * 80000, SDS-PAGE | Pseudomonas putida |
| 65500 | - |
component A 1 * 65500 + 1 * 50000, component B 1 * 80000, SDS-PAGE | Pseudomonas putida |
| 80000 | - |
component A 1 * 65500 + 1 * 50000, component B 1 * 80000, SDS-PAGE | Pseudomonas putida |
| 137800 | - |
calculated from amino acid sequence | Rattus norvegicus |
| 142000 | - |
2 * 142000, SDS-PAGE | Rattus norvegicus |
| 325000 | - |
gel filtration | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-oxo-L-proline + H2O + ATP | Pseudomonas putida | - |
L-glutamate + ADP + phosphate | - |
? |  |
| 5-oxo-L-proline + H2O + ATP | Rattus norvegicus | - |
L-glutamate + ADP + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | - |
- |
- |
| Rattus norvegicus | P97608 | rat | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Rattus norvegicus | - |
| cardiac muscle | - |
Rattus norvegicus | - |
| kidney | - |
Rattus norvegicus | - |
| liver | - |
Rattus norvegicus | - |
| lung | - |
Rattus norvegicus | - |
| skeletal muscle | - |
Rattus norvegicus | - |
| spleen | - |
Rattus norvegicus | - |
| testis | - |
Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-oxo-L-proline + H2O + ATP | - |
Pseudomonas putida | L-glutamate + ADP + phosphate | - |
? | |
| 5-oxo-L-proline + H2O + ATP | - |
Rattus norvegicus | L-glutamate + ADP + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 142000, SDS-PAGE | Rattus norvegicus |
| polymer | component A 1 * 65500 + 1 * 50000, component B 1 * 80000, SDS-PAGE | Pseudomonas putida |
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