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Literature summary for 3.5.2.9 extracted from

  • Van Der Werf, P.; Orlowski, M.; Meister, A.
    Enzymatic conversion of 5-oxo-L-proline (L-pyrrolidone carboxylate) to L-glutamate coupled with cleavage of adenosine triphosphate to adenosine diphosphate, a reaction in the gamma-glutamyl cycle (1971), Proc. Natl. Acad. Sci. USA, 68, 2982-2985.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.2.9

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 MgATP2-
-
 Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
K+ requires monovalent cation, K+ or NH4+ Rattus norvegicus
Mg2+
-
 Rattus norvegicus
Mn2+
-
 Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5-oxo-L-proline + H2O + ATP Homo sapiens
-
 L-glutamate + ADP + phosphate
-
 ?
5-oxo-L-proline + H2O + ATP Rattus norvegicus
-
 L-glutamate + ADP + phosphate
-
 ?
5-oxo-L-proline + H2O + ATP Sus scrofa
-
 L-glutamate + ADP + phosphate
-
 ?
5-oxo-L-proline + H2O + ATP Ovis aries
-
 L-glutamate + ADP + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 human
-
Ovis aries
-
 sheep
-
Rattus norvegicus
-
 rat
-
Sus scrofa
-
 pig
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Homo sapiens
-
brain
-
 Rattus norvegicus
-
brain
-
 Sus scrofa
-
cardiac muscle
-
 Rattus norvegicus
-
cardiac muscle
-
 Sus scrofa
-
intestine
-
 Rattus norvegicus
-
kidney
-
 Homo sapiens
-
kidney
-
 Rattus norvegicus
-
kidney
-
 Sus scrofa
-
kidney
-
 Ovis aries
-
liver
-
 Homo sapiens
-
liver
-
 Rattus norvegicus
-
liver
-
 Sus scrofa
-
lung
-
 Homo sapiens
-
lung
-
 Rattus norvegicus
-
spleen
-
 Rattus norvegicus
-

Storage Stability

Storage Stability Organism
4°C, loses progressively activity, 60% initial activity lost in 6 days, activity completely restored upon addition of 5 mM dithiothreitol Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-oxo-L-proline + H2O + ATP
-
 Homo sapiens L-glutamate + ADP + phosphate
-
 ?
5-oxo-L-proline + H2O + ATP
-
 Rattus norvegicus L-glutamate + ADP + phosphate
-
 ?
5-oxo-L-proline + H2O + ATP
-
 Sus scrofa L-glutamate + ADP + phosphate
-
 ?
5-oxo-L-proline + H2O + ATP
-
 Ovis aries L-glutamate + ADP + phosphate
-
 ?
MgATP2- + H2O
-
 Rattus norvegicus ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
-
 Rattus norvegicus