Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-imidazolidone-4-carboxylate | 5-oxoproline analog, stimulates rapid ATP cleavage, but is not hydrolyzed itself | Rattus norvegicus | |
2-piperidone-6-carboxylate | stimulates ATP cleavage | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.008 | - |
5-oxo-L-proline | - |
Rattus norvegicus | |
0.13 | - |
ITP | - |
Rattus norvegicus | |
0.13 | - |
ATP | measured in absence of 5-oxoproline | Rattus norvegicus | |
0.17 | - |
ATP | - |
Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
142000 | - |
2 * 142000, SDS-PAGE | Rattus norvegicus |
325000 | - |
gel filtration | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-oxo-L-proline + H2O + ATP | Rattus norvegicus | conversion essentially irreversible, very slow reversal can be shown by measurement of ATP formation in presence of high glutamate concentrations | L-glutamate + ADP + phosphate | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
rat | - |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
87 | - |
- |
Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-oxo-L-proline + H2O + ATP | - |
Rattus norvegicus | L-glutamate + ADP + phosphate | - |
ir | |
5-oxo-L-proline + H2O + ATP | conversion essentially irreversible, very slow reversal can be shown by measurement of ATP formation in presence of high glutamate concentrations | Rattus norvegicus | L-glutamate + ADP + phosphate | - |
ir | |
alpha-hydroxyglutarate lactone + H2O | - |
Rattus norvegicus | ? | - |
? | |
ATP + H2O | - |
Rattus norvegicus | ? | - |
? | |
ITP + H2O | - |
Rattus norvegicus | ? | - |
? | |
L-2-oxothiazolidine-4-carboxylate + H2O + ATP | - |
Rattus norvegicus | L-cysteine + ADP + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 142000, SDS-PAGE | Rattus norvegicus |