Cloned (Comment) | Organism |
---|---|
gene SMB-1, recombinant expression of Hhis-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS | Serratia marcescens |
Crystallization (Comment) | Organism |
---|---|
purified recombinant His-tagged enzyme, method screening, sitting drop vapour diffusion method, mixing of 0.001 ml protein solution with 0.001 ml reservoir solution containing 0.2 M ammonium sulfate, 0.1 M sodium acetate trihydrate, pH 5.3, 28% w/v PEG MME 2000, or 1.0 M lithium chloride, 0.1 M MES, pH 5.8, and 26% w/v PEG 6000, 1 week, 20°C, resulting in two different types of crystals: SMB-1a and SMB-1b, X-ray diffraction structure determination and analysis at 1.60-1.63 A resolution, molecular replacement and modeling | Serratia marcescens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Serratia marcescens | - |
gene SMB-1 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography and two different steps of gel filtration, followed by diafiltration | Serratia marcescens |
Synonyms | Comment | Organism |
---|---|---|
SMB-1 | - |
Serratia marcescens |
subclass B3 metallo-beta-lactamase | - |
Serratia marcescens |
General Information | Comment | Organism |
---|---|---|
physiological function | subclass B3 metallo-beta-lactamase SMB-1 that confers carbapenem resistance | Serratia marcescens |