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Literature summary for 3.5.2.6 extracted from

  • Wachino, J.; Yamaguchi, Y.; Mori, S.; Yamagata, Y.; Arakawa, Y.; Shibayama, K.
    Crystallization and preliminary X-ray analysis of the subclass B3 metallo-beta-lactamase SMB-1 that confers carbapenem resistance (2012), Acta Crystallogr. Sect. F, 68, 343-346.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene SMB-1, recombinant expression of Hhis-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS Serratia marcescens

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant His-tagged enzyme, method screening, sitting drop vapour diffusion method, mixing of 0.001 ml protein solution with 0.001 ml reservoir solution containing 0.2 M ammonium sulfate, 0.1 M sodium acetate trihydrate, pH 5.3, 28% w/v PEG MME 2000, or 1.0 M lithium chloride, 0.1 M MES, pH 5.8, and 26% w/v PEG 6000, 1 week, 20°C, resulting in two different types of crystals: SMB-1a and SMB-1b, X-ray diffraction structure determination and analysis at 1.60-1.63 A resolution, molecular replacement and modeling Serratia marcescens

Organism

Organism UniProt Comment Textmining
Serratia marcescens
-
gene SMB-1
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography and two different steps of gel filtration, followed by diafiltration Serratia marcescens

Synonyms

Synonyms Comment Organism
SMB-1
-
Serratia marcescens
subclass B3 metallo-beta-lactamase
-
Serratia marcescens

General Information

General Information Comment Organism
physiological function subclass B3 metallo-beta-lactamase SMB-1 that confers carbapenem resistance Serratia marcescens