Protein Variants | Comment | Organism |
---|---|---|
E166C | mutant constructed to obtain an engineered beta-lactamase that contains an environment-sensitive fluorophore conjugated near its active site to probe the structural dynamics of the omega-loop and to detect the binding of substrates. Mutant enzyme carrying a 6-bromoacetyl-2-dimethylaminonaphthalene label shows improved binding kinetics and positive fluorescence signal toward oxyimino-cephalosporins, but shows little such effect to non-oxyimino-cephalosporins. The omega-loop adopts a less stabilized structure, and readily undergoes conformational change to accommodate the binding of bulky oxyimino-cephalosporins while no such change is observed for non-oxyimino-cephalosporins | Bacillus licheniformis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0079 | - |
penicillin G | mutant E166C, carrying 6-bromoacetyl-2-dimethylaminonaphthalene label, pH 7.0, 22°C | Bacillus licheniformis | |
0.048 | - |
penicillin G | wild-type, pH 7.0, 22°C | Bacillus licheniformis | |
0.072 | - |
penicillin G | mutant E166C, pH 7.0, 22°C | Bacillus licheniformis | |
0.085 | - |
cefuroxime | wild-type, pH 7.0, 22°C | Bacillus licheniformis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus licheniformis | P00808 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cefuroxime + H2O | - |
Bacillus licheniformis | (2R)-5-[(carbamoyloxy)methyl]-2-[(R)-carboxy{[(2Z)-2-(furan-2-yl)-2-(methoxyimino)acetyl]amino}methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
penicillin G + H2O | - |
Bacillus licheniformis | (2R,4S)-2-[(R)-carboxy[(phenylacetyl)amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PenP | - |
Bacillus licheniformis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
cefuroxime | wild-type, pH 7.0, 22°C | Bacillus licheniformis | |
0.32 | - |
penicillin G | mutant E166C, carrying 6-bromoacetyl-2-dimethylaminonaphthalene label, pH 7.0, 22°C | Bacillus licheniformis | |
2.1 | - |
penicillin G | mutant E166C, pH 7.0, 22°C | Bacillus licheniformis | |
2612 | - |
penicillin G | wild-type, pH 7.0, 22°C | Bacillus licheniformis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
cefuroxime | wild-type, pH 7.0, 22°C | Bacillus licheniformis | |
29 | - |
penicillin G | mutant E166C, pH 7.0, 22°C | Bacillus licheniformis | |
40 | - |
penicillin G | mutant E166C, carrying 6-bromoacetyl-2-dimethylaminonaphthalene label, pH 7.0, 22°C | Bacillus licheniformis | |
54000 | - |
penicillin G | wild-type, pH 7.0, 22°C | Bacillus licheniformis |