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Literature summary for 3.5.2.6 extracted from

  • Badarau, A.; Page, M.I.
    Loss of enzyme activity during turnover of the Bacillus cereus beta -lactamase catalyzed hydrolysis of beta -lactams due to loss of zinc ion (2008), J. Biol. Inorg. Chem., 13, 919-928.
    View publication on PubMed
Search for more literature for 3.5.2.6

Protein Variants

Protein Variants Comment Organism
C221S mutant catalytic activity on the burst phase is similar to that of the wild type BcII Bacillus cereus

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ metalloenzyme Bacillus cereus
additional information two metal ions are required for the hydrolytic activity of native zinc class B1 MBL BcII and highlight the importance of the Zn2 site during turnover Bacillus cereus
Zn2+ metalloenzyme, dependent, exist either as mononuclear or as dinuclear enzymes Bacillus cereus

Organism

Organism UniProt Comment Textmining
Bacillus cereus P04190 569/H/9
-
Bacillus cereus 569/H/9 P04190 569/H/9
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzylpenicillin + H2O
-
 Bacillus cereus (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
benzylpenicillin + H2O
-
 Bacillus cereus 569/H/9 (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
cefuroxime + H2O
-
 Bacillus cereus (2R)-5-[(carbamoyloxy)methyl]-2-[(R)-carboxy{[(2Z)-2-(furan-2-yl)-2-(methoxyimino)acetyl]amino}methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
cefuroxime + H2O
-
 Bacillus cereus 569/H/9 (2R)-5-[(carbamoyloxy)methyl]-2-[(R)-carboxy{[(2Z)-2-(furan-2-yl)-2-(methoxyimino)acetyl]amino}methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?

Synonyms

Synonyms Comment Organism
apo Bacillus cereus 569/H/9 enzyme metal-free BcII Bacillus cereus
BcII
-
 Bacillus cereus
beta-lactamase
-
 Bacillus cereus
metallo-beta-lactamase
-
 Bacillus cereus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Bacillus cereus