Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli TG1 | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
R276C | site-directed mutagenesis, the MICs of cefotaxime are clearly lower, clearly reduced catalytic efficiency towards cefotaxime. Investigation of the role of Arg276 in cefotaxime hydrolysis | Escherichia coli |
R276G | site-directed mutagenesis, the MICs of cefotaxime are clearly lower, clearly reduced catalytic efficiency towards cefotaxime. Investigation of the role of Arg276 in cefotaxime hydrolysis | Escherichia coli |
R276H | site-directed mutagenesis, investigation of the role of Arg276 in cefotaxime hydrolysis | Escherichia coli |
R276N | site-directed mutagenesis, investigation of the role of Arg276 in cefotaxime hydrolysis | Escherichia coli |
R276S | site-directed mutagenesis, the MICs of cefotaxime are clearly lower, clearly reduced catalytic efficiency towards cefotaxime. Investigation of the role of Arg276 in cefotaxime hydrolysis | Escherichia coli |
R276W | site-directed mutagenesis, the MICs of cefotaxime are clearly lower, clearly reduced catalytic efficiency towards cefotaxime. Investigation of the role of Arg276 in cefotaxime hydrolysis | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
clavulanic acid | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.118 | - |
cefotaxime | R276G mutant, 25°C | Escherichia coli | |
0.133 | - |
cefotaxime | R276H mutant, 25°C | Escherichia coli | |
0.143 | - |
cefotaxime | R276S mutant, 25°C | Escherichia coli | |
0.153 | - |
cefotaxime | R276W mutant, 25°C | Escherichia coli | |
0.157 | - |
cefotaxime | CTX-M-1 wild type, 25°C | Escherichia coli | |
0.163 | - |
cefotaxime | R276N mutant, 25°C | Escherichia coli | |
0.163 | - |
cefalotin | R276N mutant, 25°C | Escherichia coli | |
0.206 | - |
cefalotin | R276W mutant, 25°C | Escherichia coli | |
0.207 | - |
cefalotin | R276G mutant, 25°C | Escherichia coli | |
0.22 | - |
cefotaxime | R276C mutant, 25°C | Escherichia coli | |
0.28 | - |
cefalotin | R276H mutant, 25°C | Escherichia coli | |
0.298 | - |
cefalotin | R276C mutant, 25°C | Escherichia coli | |
0.35 | - |
cefalotin | R276S mutant, 25°C | Escherichia coli | |
0.433 | - |
cefalotin | CTX-M-1 wild type, 25°C | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
SDS-PAGE | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P28585 | - |
- |
Purification (Comment) | Organism |
---|---|
CTX-M-1 wild-type and six derived mutants are purified by affinity chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cefalotin + H2O | - |
Escherichia coli | (2R)-5-[(acetyloxy)methyl]-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
cefotaxime + H2O | - |
Escherichia coli | (2R)-5-[(acetyloxy)methyl]-2-[(R)-[[(2E)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
nitrocefin + H2O | - |
Escherichia coli | (2R)-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 28000, SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
CTX-M beta-lactamase | - |
Escherichia coli |
CTX-M-1 | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
21 | - |
cefotaxime | R276G mutant, 25°C | Escherichia coli | |
44 | - |
cefotaxime | R276S mutant, 25°C | Escherichia coli | |
91 | - |
cefotaxime | R276C mutant, 25°C | Escherichia coli | |
92 | - |
cefotaxime | R276W mutant, 25°C | Escherichia coli | |
107 | - |
cefotaxime | R276N mutant, 25°C | Escherichia coli | |
120 | - |
cefotaxime | R276H mutant, 25°C | Escherichia coli | |
140 | - |
cefotaxime | CTX-M-1 wild type, 25°C | Escherichia coli | |
630 | - |
cefalotin | R276H mutant, 25°C | Escherichia coli | |
680 | - |
cefalotin | R276G mutant, 25°C | Escherichia coli | |
700 | - |
cefalotin | R276C mutant, 25°C | Escherichia coli | |
1180 | - |
cefalotin | R276W mutant, 25°C | Escherichia coli | |
1190 | - |
cefalotin | CTX-M-1 wild type, 25°C | Escherichia coli | |
1290 | - |
cefalotin | R276N mutant, 25°C | Escherichia coli | |
1800 | - |
cefalotin | R276S mutant, 25°C | Escherichia coli |