Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.5.2.6 extracted from

  • Perez-Llarena, F.J.; Cartelle, M.; Mallo, S.; Beceiro, A.; Perez, A.; Villanueva, R.; Romero, A.; Bonnet, R.; Bou, G.
    Structure-function studies of arginine at position 276 in CTX-M beta -lactamases (2008), J. Antimicrob. Chemother., 61, 792-797.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli TG1 Escherichia coli

Protein Variants

Protein Variants Comment Organism
R276C site-directed mutagenesis, the MICs of cefotaxime are clearly lower, clearly reduced catalytic efficiency towards cefotaxime. Investigation of the role of Arg276 in cefotaxime hydrolysis Escherichia coli
R276G site-directed mutagenesis, the MICs of cefotaxime are clearly lower, clearly reduced catalytic efficiency towards cefotaxime. Investigation of the role of Arg276 in cefotaxime hydrolysis Escherichia coli
R276H site-directed mutagenesis, investigation of the role of Arg276 in cefotaxime hydrolysis Escherichia coli
R276N site-directed mutagenesis, investigation of the role of Arg276 in cefotaxime hydrolysis Escherichia coli
R276S site-directed mutagenesis, the MICs of cefotaxime are clearly lower, clearly reduced catalytic efficiency towards cefotaxime. Investigation of the role of Arg276 in cefotaxime hydrolysis Escherichia coli
R276W site-directed mutagenesis, the MICs of cefotaxime are clearly lower, clearly reduced catalytic efficiency towards cefotaxime. Investigation of the role of Arg276 in cefotaxime hydrolysis Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
clavulanic acid
-
Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.118
-
cefotaxime R276G mutant, 25°C Escherichia coli
0.133
-
cefotaxime R276H mutant, 25°C Escherichia coli
0.143
-
cefotaxime R276S mutant, 25°C Escherichia coli
0.153
-
cefotaxime R276W mutant, 25°C Escherichia coli
0.157
-
cefotaxime CTX-M-1 wild type, 25°C Escherichia coli
0.163
-
cefotaxime R276N mutant, 25°C Escherichia coli
0.163
-
cefalotin R276N mutant, 25°C Escherichia coli
0.206
-
cefalotin R276W mutant, 25°C Escherichia coli
0.207
-
cefalotin R276G mutant, 25°C Escherichia coli
0.22
-
cefotaxime R276C mutant, 25°C Escherichia coli
0.28
-
cefalotin R276H mutant, 25°C Escherichia coli
0.298
-
cefalotin R276C mutant, 25°C Escherichia coli
0.35
-
cefalotin R276S mutant, 25°C Escherichia coli
0.433
-
cefalotin CTX-M-1 wild type, 25°C Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
SDS-PAGE Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P28585
-
-

Purification (Commentary)

Purification (Comment) Organism
CTX-M-1 wild-type and six derived mutants are purified by affinity chromatography Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cefalotin + H2O
-
Escherichia coli (2R)-5-[(acetyloxy)methyl]-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
?
cefotaxime + H2O
-
Escherichia coli (2R)-5-[(acetyloxy)methyl]-2-[(R)-[[(2E)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
?
nitrocefin + H2O
-
Escherichia coli (2R)-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
?

Subunits

Subunits Comment Organism
? x * 28000, SDS-PAGE Escherichia coli

Synonyms

Synonyms Comment Organism
CTX-M beta-lactamase
-
Escherichia coli
CTX-M-1
-
Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
21
-
cefotaxime R276G mutant, 25°C Escherichia coli
44
-
cefotaxime R276S mutant, 25°C Escherichia coli
91
-
cefotaxime R276C mutant, 25°C Escherichia coli
92
-
cefotaxime R276W mutant, 25°C Escherichia coli
107
-
cefotaxime R276N mutant, 25°C Escherichia coli
120
-
cefotaxime R276H mutant, 25°C Escherichia coli
140
-
cefotaxime CTX-M-1 wild type, 25°C Escherichia coli
630
-
cefalotin R276H mutant, 25°C Escherichia coli
680
-
cefalotin R276G mutant, 25°C Escherichia coli
700
-
cefalotin R276C mutant, 25°C Escherichia coli
1180
-
cefalotin R276W mutant, 25°C Escherichia coli
1190
-
cefalotin CTX-M-1 wild type, 25°C Escherichia coli
1290
-
cefalotin R276N mutant, 25°C Escherichia coli
1800
-
cefalotin R276S mutant, 25°C Escherichia coli