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Literature summary for 3.5.2.6 extracted from
- Conformational changes in the metallo-beta -lactamase ImiS during the catalytic reaction: An EPR spectrokinetic study of Co(II)-spin label interactions (2008), J. Am. Chem. Soc., 130, 8215-8222.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) | Aeromonas veronii bv. sobria |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E152C | ImiS mutant (ImiS[E152C]) is generated by mutating the imiS gene in pET26bimiS. Mutant exhibits very similar kinetic behavior to wild type | Aeromonas veronii bv. sobria |
| spin-labeled ImiS | ImiS* | Aeromonas veronii bv. sobria |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.104 | - |
Imipenem | spin-labeled enzyme, pH 7.0, 25°C | Aeromonas veronii bv. sobria |  |
| 0.106 | - |
Imipenem | E152C mutant, pH 7.0, 25°C | Aeromonas veronii bv. sobria | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | metalloenzyme | Aeromonas veronii bv. sobria | |
| Zn2+ | metalloenzyme, E152C mutant contains 0.80 equivalents of Zn, which is similar to the metal content of wild type enzyme | Aeromonas veronii bv. sobria | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Aeromonas veronii bv. sobria | readily hydrolyzes carbapenems | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aeromonas veronii bv. sobria | O31272 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| E152C mutant is purified | Aeromonas veronii bv. sobria |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| imipenem + H2O | - |
Aeromonas veronii bv. sobria | (5R)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-({2-[(iminomethyl)amino]ethyl}sulfanyl)-4,5-dihydro-1H-pyrrole-2-carboxylic acid | - |
? | |
| additional information | readily hydrolyzes carbapenems | Aeromonas veronii bv. sobria | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ImiS | - |
Aeromonas veronii bv. sobria |
| MbetaL | - |
Aeromonas veronii bv. sobria |
| metallo-beta-lactamase | - |
Aeromonas veronii bv. sobria |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Aeromonas veronii bv. sobria |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 292 | - |
Imipenem | E152C mutant, pH 7.0, 25°C | Aeromonas veronii bv. sobria | |
| 295 | - |
Imipenem | spin-labeled enzyme, pH 7.0, 25°C | Aeromonas veronii bv. sobria | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Aeromonas veronii bv. sobria |
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