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Literature summary for 3.5.2.6 extracted from

  • Wang, Q.; Cheng, J.; Chen, Y.; Ye, Y.; Li, J.; Zhang, X.
    Characterization of a novel AmpC-type plasmid-mediated beta -lactamase from an Escherichia coli strain isolated in China (2008), Curr. Microbiol., 57, 558-563.
    View publication on PubMed
Search for more literature for 3.5.2.6

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
clavulanic acid
-
 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.022
-
 penicillin G 30°C, pH 7.0 Escherichia coli
0.029
-
 cefotaxime 30°C, pH 7.0 Escherichia coli
0.04
-
 cefuroxime 30°C, pH 7.0 Escherichia coli
0.044
-
 cefoxitin 30°C, pH 7.0 Escherichia coli
0.062
-
 cephalothin 30°C, pH 7.0 Escherichia coli
0.115
-
 ceftazidime 30°C, pH 7.0. The affinity of MIR-4 beta-lactamase for ceftazidime is quite low Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 SDS-PAGE Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli A3RIY0 E384
-
Escherichia coli E384 A3RIY0 E384
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cefotaxime + H2O
-
 Escherichia coli (2R)-5-[(acetyloxy)methyl]-2-[(R)-[[(2E)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
cefotaxime + H2O
-
 Escherichia coli E384 (2R)-5-[(acetyloxy)methyl]-2-[(R)-[[(2E)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
cefoxitin + H2O
-
 Escherichia coli (2R)-5-[(carbamoyloxy)methyl]-2-[(S)-carboxy(methoxy)[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
cefoxitin + H2O
-
 Escherichia coli E384 (2R)-5-[(carbamoyloxy)methyl]-2-[(S)-carboxy(methoxy)[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
ceftazidime + H2O 30°C, pH 7.0. The affinity of MIR-4 beta-lactamase for ceftazidime is quite low Escherichia coli (2R)-2-[(R)-{[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-{[(2-carboxypropan-2-yl)oxy]imino}acetyl]amino}(carboxy)methyl]-5-[(pyridin-1-ium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
ceftazidime + H2O 30°C, pH 7.0. The affinity of MIR-4 beta-lactamase for ceftazidime is quite low Escherichia coli E384 (2R)-2-[(R)-{[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-{[(2-carboxypropan-2-yl)oxy]imino}acetyl]amino}(carboxy)methyl]-5-[(pyridin-1-ium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
cefuroxime + H2O
-
 Escherichia coli (2R)-5-[(carbamoyloxy)methyl]-2-[(R)-carboxy{[(2Z)-2-(furan-2-yl)-2-(methoxyimino)acetyl]amino}methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
cefuroxime + H2O
-
 Escherichia coli E384 (2R)-5-[(carbamoyloxy)methyl]-2-[(R)-carboxy{[(2Z)-2-(furan-2-yl)-2-(methoxyimino)acetyl]amino}methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
cephalosporin + H2O
-
 Escherichia coli ?
-
 ?
cephalothin + H2O
-
 Escherichia coli (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
cephalothin + H2O
-
 Escherichia coli E384 (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
penicillin G + H2O
-
 Escherichia coli (2R,4S)-2-[(R)-carboxy[(phenylacetyl)amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?

Subunits

Subunits Comment Organism
? x * 40000, SDS-PAGE Escherichia coli

Synonyms

Synonyms Comment Organism
beta-lactamase AmpC-type plasmid-mediated Escherichia coli
MIR-4 beta-lactamase
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Escherichia coli

pI Value

Organism Comment pI Value Maximum pI Value
Escherichia coli isoelectric focusing
-
 8.2