Literature summary for 3.5.2.6 extracted from

Beceiro, A.; Perez-Llarena, F.-J.; Perez, A.; Tomas, M.; Fernandez, A.; Mallo, S.; Villanueva, R.; Bou, G.
Molecular characterization of the gene encoding a new AmpC beta-lactamase in Acinetobacter baylyi (2007), J. Antimicrob. Chemother., 59, 996-1000.

Search for more literature for 3.5.2.6

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli	Acinetobacter baylyi

Inhibitors

Inhibitors	Comment	Organism	Structure
clavulanic acid	-	Acinetobacter baylyi
Sulbactam	-	Acinetobacter baylyi

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00613	-	cefalotin	-	Acinetobacter baylyi
0.00895	-	ampicillin	-	Acinetobacter baylyi

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
40000	-	SDS-PAGE	Acinetobacter baylyi

Organism

Organism	UniProt	Comment	Textmining
Acinetobacter baylyi	Q0VJU2	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.0216	-	using nitrocefin as a substrate	Acinetobacter baylyi

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ampicillin + H2O	-	Acinetobacter baylyi	(2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid	-	?
cefalotin + H2O	-	Acinetobacter baylyi	(2R)-5-[(acetyloxy)methyl]-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid	-	?
nitrocefin + H2O	-	Acinetobacter baylyi	(2R)-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid	-	?

Synonyms

Synonyms	Comment	Organism
AmpC	-	Acinetobacter baylyi

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.17	-	ampicillin	-	Acinetobacter baylyi
4.22	-	cefalotin	-	Acinetobacter baylyi

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.0139	-	-	Acinetobacter baylyi	Sulbactam
0.25	-	IC50 above 0.25 mM	Acinetobacter baylyi	clavulanic acid

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Release 2023.1 (January 2023)