Literature summary for 3.5.2.6 extracted from

Michaux, C.; Massant, J.; Kerff, F.; Frere, J.M.; Docquier, J.D.; Vandenberghe, I.; Samyn, B.; Pierrard, A.; Feller, G.; Charlier, P.; Van Beeumen, J.; Wouters, J.
Crystal structure of a cold-adapted class C beta-lactamase (2008), FEBS J., 275, 1687-1697.

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapour diffusion method with 20% poly(ethylene)glycol 6 K in 0.1 M Tris (pH 8.0)	Serratia marcescens
hanging drop vapour diffusion method with 20% poly(ethylene)glycol 6 K in 0.1 M Tris (pH 8.0)	Psychrobacter immobilis
hanging drop vapour diffusion method with 20% poly(ethylene)glycol 6 K in 0.1 M Tris (pH 8.0)	Pseudomonas fluorescens
hanging drop vapour diffusion method with 20% poly(ethylene)glycol 6 K in 0.1 M Tris (pH 8.0)	Enterobacter cloacae

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
0.0000005	-	Cloxacillin	at 30°C in 50mM sodium phosphate buffer (pH 7.0)	Enterobacter cloacae
0.00001	-	ampicillin	at 30°C in 50 mM sodium phosphate buffer (pH 7.0)	Serratia marcescens
0.000118	-	Oxacillin	at 30°C in 50mM sodium phosphate buffer (pH 7.0)	Pseudomonas fluorescens
0.00016	-	Cloxacillin	at 30°C in 50mM sodium phosphate buffer (pH 7.0)	Pseudomonas fluorescens
0.0005	-	benzylpenicillin	at 30°C in 50mM sodium phosphate buffer (pH 7.0)	Enterobacter cloacae
0.0017	-	benzylpenicillin	at 30°C in 50 mM sodium phosphate buffer (pH 7.0)	Serratia marcescens
0.0017	-	benzylpenicillin	at 30°C in 50mM sodium phosphate buffer (pH 7.0)	Psychrobacter immobilis
0.0023	-	benzylpenicillin	at 30°C in 50mM sodium phosphate buffer (pH 7.0)	Pseudomonas fluorescens
0.0037	-	carbenicillin	at 30°C in 50mM sodium phosphate buffer (pH 7.0)	Pseudomonas fluorescens
0.004	-	ampicillin	at 30°C in 50mM sodium phosphate buffer (pH 7.0)	Enterobacter cloacae
0.0051	-	ampicillin	at 30°C in 50mM sodium phosphate buffer (pH 7.0)	Pseudomonas fluorescens
0.006	-	ampicillin	at 30°C in 50mM sodium phosphate buffer (pH 7.0)	Psychrobacter immobilis
0.008	-	cephalexin	at 30°C in 50mM sodium phosphate buffer (pH 7.0)	Psychrobacter immobilis
0.0085	-	cephalexin	at 30°C in 50mM sodium phosphate buffer (pH 7.0)	Enterobacter cloacae
0.023	-	nitrocefin	at 30°C in 50mM sodium phosphate buffer (pH 7.0)	Enterobacter cloacae
0.03	-	cephalexin	at 30°C in 50 mM sodium phosphate buffer (pH 7.0)	Serratia marcescens
0.04	-	nitrocefin	at 30°C in 50 mM sodium phosphate buffer (pH 7.0)	Serratia marcescens
0.05	-	cephalexin	at 30°C in 50mM sodium phosphate buffer (pH 7.0)	Pseudomonas fluorescens
0.051	-	nitrocefin	at 30°C in 50mM sodium phosphate buffer (pH 7.0)	Psychrobacter immobilis
0.1	-	cefazolin	at 30°C in 50mM sodium phosphate buffer (pH 7.0)	Psychrobacter immobilis
0.116	-	nitrocefin	at 30°C in 50mM sodium phosphate buffer (pH 7.0)	Pseudomonas fluorescens
0.15	-	cefazolin	at 30°C in 50mM sodium phosphate buffer (pH 7.0)	Pseudomonas fluorescens
0.54	-	cefazolin	at 30°C in 50 mM sodium phosphate buffer (pH 7.0)	Serratia marcescens
1.5	-	cefazolin	at 30°C in 50mM sodium phosphate buffer (pH 7.0)	Enterobacter cloacae

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
38720	-	MALDI-TOF mass spectrometry	Serratia marcescens
38720	-	MALDI-TOF mass spectrometry	Psychrobacter immobilis
38720	-	MALDI-TOF mass spectrometry	Pseudomonas fluorescens
38720	-	MALDI-TOF mass spectrometry	Enterobacter cloacae

Organism

Organism	UniProt	Comment	Textmining
Enterobacter cloacae	P05364	-	-
Enterobacter cloacae 908R	P05364	-	-
Pseudomonas fluorescens	P85302	-	-
Pseudomonas fluorescens TAE4	P85302	-	-
Psychrobacter immobilis	-	-	-
Serratia marcescens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Serratia marcescens
-	Psychrobacter immobilis
-	Pseudomonas fluorescens
-	Enterobacter cloacae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
ampicillin + H2O	-	Serratia marcescens	(2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid	-	?
ampicillin + H2O	-	Psychrobacter immobilis	(2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid	-	?
ampicillin + H2O	-	Pseudomonas fluorescens	(2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid	-	?
ampicillin + H2O	-	Enterobacter cloacae	(2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid	-	?
ampicillin + H2O	-	Pseudomonas fluorescens TAE4	(2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid	-	?
ampicillin + H2O	-	Enterobacter cloacae 908R	(2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid	-	?
benzylpenicillin + H2O	-	Serratia marcescens	(2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid	-	?
benzylpenicillin + H2O	-	Psychrobacter immobilis	(2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid	-	?
benzylpenicillin + H2O	-	Pseudomonas fluorescens	(2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid	-	?
benzylpenicillin + H2O	-	Enterobacter cloacae	(2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid	-	?
benzylpenicillin + H2O	-	Pseudomonas fluorescens TAE4	(2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid	-	?
benzylpenicillin + H2O	-	Enterobacter cloacae 908R	(2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid	-	?
carbenicillin + H2O	-	Serratia marcescens	(2R,4S)-2-{(R)-carboxy[2-carboxy(phenyl)acetamido]methyl}-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid	-	?
carbenicillin + H2O	-	Psychrobacter immobilis	(2R,4S)-2-{(R)-carboxy[2-carboxy(phenyl)acetamido]methyl}-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid	-	?
carbenicillin + H2O	-	Pseudomonas fluorescens	(2R,4S)-2-{(R)-carboxy[2-carboxy(phenyl)acetamido]methyl}-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid	-	?
carbenicillin + H2O	-	Enterobacter cloacae	(2R,4S)-2-{(R)-carboxy[2-carboxy(phenyl)acetamido]methyl}-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid	-	?
carbenicillin + H2O	-	Pseudomonas fluorescens TAE4	(2R,4S)-2-{(R)-carboxy[2-carboxy(phenyl)acetamido]methyl}-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid	-	?
carbenicillin + H2O	-	Enterobacter cloacae 908R	(2R,4S)-2-{(R)-carboxy[2-carboxy(phenyl)acetamido]methyl}-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid	-	?
cefazolin + H2O	-	Serratia marcescens	(2R)-2-[(R)-carboxy[(1H-tetrazol-1-ylacetyl)amino]methyl]-5-[[(5-methyl-1,3,4-thiadiazol-2-yl)sulfanyl]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid	-	?
cefazolin + H2O	-	Psychrobacter immobilis	(2R)-2-[(R)-carboxy[(1H-tetrazol-1-ylacetyl)amino]methyl]-5-[[(5-methyl-1,3,4-thiadiazol-2-yl)sulfanyl]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid	-	?
cefazolin + H2O	-	Pseudomonas fluorescens	(2R)-2-[(R)-carboxy[(1H-tetrazol-1-ylacetyl)amino]methyl]-5-[[(5-methyl-1,3,4-thiadiazol-2-yl)sulfanyl]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid	-	?
cefazolin + H2O	-	Enterobacter cloacae	(2R)-2-[(R)-carboxy[(1H-tetrazol-1-ylacetyl)amino]methyl]-5-[[(5-methyl-1,3,4-thiadiazol-2-yl)sulfanyl]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid	-	?
cephalexin + H2O	-	Serratia marcescens	(2R)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5-methyl-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid	-	?
cephalexin + H2O	-	Psychrobacter immobilis	(2R)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5-methyl-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid	-	?
cephalexin + H2O	-	Pseudomonas fluorescens	(2R)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5-methyl-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid	-	?
cephalexin + H2O	-	Enterobacter cloacae	(2R)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5-methyl-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid	-	?
cephalexin + H2O	-	Pseudomonas fluorescens TAE4	(2R)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5-methyl-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid	-	?
cephalexin + H2O	-	Enterobacter cloacae 908R	(2R)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5-methyl-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid	-	?
cloxacillin + H2O	-	Serratia marcescens	(2R,4S)-2-[(R)-carboxy{[3-(2-chlorophenyl)-5-methyl-1,2-oxazole-4-carbonyl]amino}methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid	-	?
cloxacillin + H2O	-	Psychrobacter immobilis	(2R,4S)-2-[(R)-carboxy{[3-(2-chlorophenyl)-5-methyl-1,2-oxazole-4-carbonyl]amino}methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid	-	?
cloxacillin + H2O	-	Pseudomonas fluorescens	(2R,4S)-2-[(R)-carboxy{[3-(2-chlorophenyl)-5-methyl-1,2-oxazole-4-carbonyl]amino}methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid	-	?
cloxacillin + H2O	-	Enterobacter cloacae	(2R,4S)-2-[(R)-carboxy{[3-(2-chlorophenyl)-5-methyl-1,2-oxazole-4-carbonyl]amino}methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid	-	?
cloxacillin + H2O	-	Pseudomonas fluorescens TAE4	(2R,4S)-2-[(R)-carboxy{[3-(2-chlorophenyl)-5-methyl-1,2-oxazole-4-carbonyl]amino}methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid	-	?
cloxacillin + H2O	-	Enterobacter cloacae 908R	(2R,4S)-2-[(R)-carboxy{[3-(2-chlorophenyl)-5-methyl-1,2-oxazole-4-carbonyl]amino}methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid	-	?
nitrocefin + H2O	-	Serratia marcescens	(2R)-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid	-	?
nitrocefin + H2O	-	Psychrobacter immobilis	(2R)-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid	-	?
nitrocefin + H2O	-	Pseudomonas fluorescens	(2R)-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid	-	?
nitrocefin + H2O	-	Enterobacter cloacae	(2R)-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid	-	?
oxacillin + H2O	-	Serratia marcescens	(2R,4S)-2-{(R)-carboxy[(5-methyl-3-phenyl-1,2-oxazole-4-carbonyl)amino]methyl}-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid	-	?
oxacillin + H2O	-	Psychrobacter immobilis	(2R,4S)-2-{(R)-carboxy[(5-methyl-3-phenyl-1,2-oxazole-4-carbonyl)amino]methyl}-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid	-	?
oxacillin + H2O	-	Pseudomonas fluorescens	(2R,4S)-2-{(R)-carboxy[(5-methyl-3-phenyl-1,2-oxazole-4-carbonyl)amino]methyl}-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid	-	?
oxacillin + H2O	-	Enterobacter cloacae	(2R,4S)-2-{(R)-carboxy[(5-methyl-3-phenyl-1,2-oxazole-4-carbonyl)amino]methyl}-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid	-	?

Synonyms

Synonyms	Comment	Organism
class C beta-lactamase	-	Serratia marcescens
class C beta-lactamase	-	Psychrobacter immobilis
class C beta-lactamase	-	Pseudomonas fluorescens
class C beta-lactamase	-	Enterobacter cloacae

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism
0.004	-	carbenicillin	at 30°C in 50mM sodium phosphate buffer (pH 7.0)	Enterobacter cloacae
0.004	-	Cloxacillin	at 30°C in 50mM sodium phosphate buffer (pH 7.0)	Enterobacter cloacae
0.008	-	Oxacillin	at 30°C in 50mM sodium phosphate buffer (pH 7.0)	Enterobacter cloacae
0.21	-	Oxacillin	at 30°C in 50mM sodium phosphate buffer (pH 7.0)	Pseudomonas fluorescens
0.42	-	Cloxacillin	at 30°C in 50mM sodium phosphate buffer (pH 7.0)	Pseudomonas fluorescens
0.46	-	ampicillin	at 30°C in 50 mM sodium phosphate buffer (pH 7.0)	Serratia marcescens
0.52	-	carbenicillin	at 30°C in 50mM sodium phosphate buffer (pH 7.0)	Pseudomonas fluorescens
0.53	-	ampicillin	at 30°C in 50mM sodium phosphate buffer (pH 7.0)	Enterobacter cloacae
0.65	-	ampicillin	at 30°C in 50mM sodium phosphate buffer (pH 7.0)	Pseudomonas fluorescens
1	-	ampicillin	at 30°C in 50mM sodium phosphate buffer (pH 7.0)	Psychrobacter immobilis
18	-	benzylpenicillin	at 30°C in 50mM sodium phosphate buffer (pH 7.0)	Enterobacter cloacae
20	-	benzylpenicillin	at 30°C in 50mM sodium phosphate buffer (pH 7.0)	Psychrobacter immobilis
26	-	cephalexin	at 30°C in 50mM sodium phosphate buffer (pH 7.0)	Psychrobacter immobilis
31	-	cephalexin	at 30°C in 50mM sodium phosphate buffer (pH 7.0)	Pseudomonas fluorescens
34.3	-	benzylpenicillin	at 30°C in 50mM sodium phosphate buffer (pH 7.0)	Pseudomonas fluorescens
61	-	cefazolin	at 30°C in 50mM sodium phosphate buffer (pH 7.0)	Pseudomonas fluorescens
72	-	cephalexin	at 30°C in 50mM sodium phosphate buffer (pH 7.0)	Enterobacter cloacae
75	-	benzylpenicillin	at 30°C in 50 mM sodium phosphate buffer (pH 7.0)	Serratia marcescens
80	-	cephalexin	at 30°C in 50 mM sodium phosphate buffer (pH 7.0)	Serratia marcescens
476	-	cefazolin	at 30°C in 50mM sodium phosphate buffer (pH 7.0)	Psychrobacter immobilis
550	-	nitrocefin	at 30°C in 50mM sodium phosphate buffer (pH 7.0)	Pseudomonas fluorescens
780	-	nitrocefin	at 30°C in 50mM sodium phosphate buffer (pH 7.0)	Enterobacter cloacae
1240	-	nitrocefin	at 30°C in 50 mM sodium phosphate buffer (pH 7.0)	Serratia marcescens
1300	-	cefazolin	at 30°C in 50 mM sodium phosphate buffer (pH 7.0)	Serratia marcescens
1407	-	nitrocefin	at 30°C in 50mM sodium phosphate buffer (pH 7.0)	Psychrobacter immobilis
3000	-	cefazolin	at 30°C in 50mM sodium phosphate buffer (pH 7.0)	Enterobacter cloacae