Crystallization (Comment) | Organism |
---|---|
crystal structure of wild-type enzyme mutant enzyme N220G, and of the complex of wild-type enzyme with biapenem | Aeromonas hydrophila |
Protein Variants | Comment | Organism |
---|---|---|
N220G | kinetic parameters of the wild-type and N220G mutant enzymes are not significantly different | Aeromonas hydrophila |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
Imipenem | 30°C, pH 6.5, mutant enzyme N220G | Aeromonas hydrophila | |
0.118 | - |
biapenem | 30°C, pH 6.5, mutant enzyme N220G | Aeromonas hydrophila | |
0.166 | - |
biapenem | 30°C, pH 6.5, wild-type enzyme | Aeromonas hydrophila | |
0.34 | - |
Imipenem | 30°C, pH 6.5, wild-type enzyme | Aeromonas hydrophila |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zinc | enzyme contains one zinc ion | Aeromonas hydrophila |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aeromonas hydrophila | P26918 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
biapenem + H2O | - |
Aeromonas hydrophila | (4R,5S)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-[(6,7-dihydro-5H-pyrazolo[1,2-a][1,2,4]triazol-4-ium-6-yl)sulfanyl]-4-methyl-4,5-dihydro-1H-pyrrole-2-carboxylate | - |
? | |
imipenem + H2O | - |
Aeromonas hydrophila | (5R)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-({2-[(iminomethyl)amino]ethyl}sulfanyl)-4,5-dihydro-1H-pyrrole-2-carboxylic acid | - |
? |
Synonyms | Comment | Organism |
---|---|---|
carbapenemase CphA | - |
Aeromonas hydrophila |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
160 | - |
biapenem | 30°C, pH 6.5, mutant enzyme N220G | Aeromonas hydrophila | |
300 | - |
biapenem | 30°C, pH 6.5, wild-type enzyme | Aeromonas hydrophila | |
390 | - |
Imipenem | 30°C, pH 6.5, mutant enzyme N220G | Aeromonas hydrophila | |
1200 | - |
Imipenem | 30°C, pH 6.5, wild-type enzyme | Aeromonas hydrophila |