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Literature summary for 3.5.2.6 extracted from

  • Paetzel, M.; Danel, F.; de Castro, L.; Mosimann, S.C.; Page, M.G.; Strynadka, N.C.
    Crystal structure of the class D beta-lactamase OXA-10 (2000), Nat. Struct. Biol., 7, 918-925.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant native and chloride-treated enzyme, from 1.8 M ammonium sulfate, 0.1 M MES, pH 6.5, 10 mM CoCl2, vapour diffusion method, X-ray diffraction structure determination and analysis at 2.0 A resolution, modeling Pseudomonas aeruginosa

Metals/Ions

Metals/Ions Comment Organism Structure
Cd2+ activates 1.65fold Pseudomonas aeruginosa
Co2+ activates 1.4fold Pseudomonas aeruginosa
Cu2+ activates 1.75fold Pseudomonas aeruginosa
KCl leads to dissociation of the dimer Pseudomonas aeruginosa
Mn2+ activates slightly, 1.12fold Pseudomonas aeruginosa
additional information no effect by Ca2+, Mg2+, K+, and EDTA Pseudomonas aeruginosa
NaCl leads to dissociation of the dimer Pseudomonas aeruginosa
Ni2+ activates 1.3fold Pseudomonas aeruginosa
Zn2+ activates 1.65fold Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27550
-
2 * 27550, sequence calculation Pseudomonas aeruginosa
53000
-
in presence of Cd2+, Cu2+ or Zn2+, gel filtration and analytical ultracentrifugation Pseudomonas aeruginosa
55100
-
analytical ultracentriguation Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa P14489 class D beta-lactamase
-

Reaction

Reaction Comment Organism Reaction ID
a beta-lactam + H2O = a substituted beta-amino acid catalytic mechanism of enzyme class D, unique in comparison with classes A and C, active site structure with elements S-X-X-K, S-X-N, and K-T/S-G Pseudomonas aeruginosa

Subunits

Subunits Comment Organism
dimer 2 * 27550, sequence calculation Pseudomonas aeruginosa
More cation mediated formation of a homodimer, but NaCl leads to dissociation of the dimer, overview Pseudomonas aeruginosa

Synonyms

Synonyms Comment Organism
OXA-10
-
Pseudomonas aeruginosa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
room temperature, assay at Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Pseudomonas aeruginosa