Crystallization (Comment) | Organism |
---|---|
purified recombinant native and chloride-treated enzyme, from 1.8 M ammonium sulfate, 0.1 M MES, pH 6.5, 10 mM CoCl2, vapour diffusion method, X-ray diffraction structure determination and analysis at 2.0 A resolution, modeling | Pseudomonas aeruginosa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | activates 1.65fold | Pseudomonas aeruginosa | |
Co2+ | activates 1.4fold | Pseudomonas aeruginosa | |
Cu2+ | activates 1.75fold | Pseudomonas aeruginosa | |
KCl | leads to dissociation of the dimer | Pseudomonas aeruginosa | |
Mn2+ | activates slightly, 1.12fold | Pseudomonas aeruginosa | |
additional information | no effect by Ca2+, Mg2+, K+, and EDTA | Pseudomonas aeruginosa | |
NaCl | leads to dissociation of the dimer | Pseudomonas aeruginosa | |
Ni2+ | activates 1.3fold | Pseudomonas aeruginosa | |
Zn2+ | activates 1.65fold | Pseudomonas aeruginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27550 | - |
2 * 27550, sequence calculation | Pseudomonas aeruginosa |
53000 | - |
in presence of Cd2+, Cu2+ or Zn2+, gel filtration and analytical ultracentrifugation | Pseudomonas aeruginosa |
55100 | - |
analytical ultracentriguation | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | P14489 | class D beta-lactamase | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a beta-lactam + H2O = a substituted beta-amino acid | catalytic mechanism of enzyme class D, unique in comparison with classes A and C, active site structure with elements S-X-X-K, S-X-N, and K-T/S-G | Pseudomonas aeruginosa |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 27550, sequence calculation | Pseudomonas aeruginosa |
More | cation mediated formation of a homodimer, but NaCl leads to dissociation of the dimer, overview | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
OXA-10 | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
room temperature, assay at | Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Pseudomonas aeruginosa |