Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutants in Escherichia coli DH10B | Klebsiella pneumoniae |
Protein Variants | Comment | Organism |
---|---|---|
S130G | site-directed mutagenesis, resistant to inhibition by taxobactam in contrast to the wild-type enzyme | Klebsiella pneumoniae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
tazobactam | class A-specific inhibitor, inactivation of the wild-type enzyme, binds to Ser70 of the active site, mutant S130G is resistant, inhibition mechanism, the mutation S130G is not essential for resistance but for acylation of the inhibitor molecule, which initiates the fragmentation of tazobactam by the mutant enzyme, mass spectrometry analysis | Klebsiella pneumoniae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella pneumoniae | - |
class A enzyme SHV-1 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
tazobactam + H2O | mutant enzyme S130G, hydrolysis of the inhibitor molecule acylated by the mutant enzyme | Klebsiella pneumoniae | (2R,4S,5S)-2-(carboxymethyl)-5-methyl-1,1-dioxo-5-[(1H-1,2,3-triazol-1-yl)methyl]-1lambda6,3-thiazolidine-4-carboxylic acid | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SHV-1 | - |
Klebsiella pneumoniae |