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Literature summary for 3.5.2.6 extracted from

  • Guo, F.; Huynh, J.; Dmitrienko, G.I.; Viswanatha, T.; Clarke, A.J.
    The role of the non-conserved residue at position 104 of class A beta-lactamases in susceptibility to mechanism-based inhibitors (1999), Biochim. Biophys. Acta, 1431, 132-147.
    View publication on PubMed
Search for more literature for 3.5.2.6

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Bacillus cereus

Protein Variants

Protein Variants Comment Organism
D104A km for benzylpenicillin and methicillin increased 2 fold, no change in catalytic activity Bacillus cereus

Inhibitors

Inhibitors Comment Organism Structure
6-beta-trifluoromethanesulfonamidopenicillanic acid
-
 Bacillus cereus
6-bromopenicillanic acid
-
 Bacillus cereus
clavulanic acid
-
 Staphylococcus aureus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Staphylococcus aureus
-
-
extracellular
-
 Bacillus cereus
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ampicillin + H2O Bacillus cereus
-
 (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
ampicillin + H2O Bacillus cereus 569/H/9
-
 (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus cereus
-
-
-
Bacillus cereus 569/H/9
-
-
-
Staphylococcus aureus
-
-
-
Staphylococcus aureus PC1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Staphylococcus aureus
-
 Bacillus cereus

Storage Stability

Storage Stability Organism
-20°C, 50 mM sodium phosphate buffer, pH 6.8, 50% glycerol Staphylococcus aureus
-20°C, 50 mM sodium phosphate buffer, pH 6.8, 50% glycerol Bacillus cereus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ampicillin + H2O
-
 Staphylococcus aureus (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
ampicillin + H2O
-
 Bacillus cereus (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
ampicillin + H2O
-
 Bacillus cereus 569/H/9 (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
ampicillin + H2O
-
 Staphylococcus aureus PC1 (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
benzylpenicillin + H2O
-
 Staphylococcus aureus (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
benzylpenicillin + H2O
-
 Bacillus cereus (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
benzylpenicillin + H2O
-
 Bacillus cereus 569/H/9 (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
benzylpenicillin + H2O
-
 Staphylococcus aureus PC1 (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
methicillin + H2O
-
 Bacillus cereus (2R,4S)-2-[(R)-carboxy(2,6-dimethoxybenzamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
methicillin + H2O
-
 Bacillus cereus 569/H/9 (2R,4S)-2-[(R)-carboxy(2,6-dimethoxybenzamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
oxacillin + H2O
-
 Staphylococcus aureus (2R,4S)-2-{(R)-carboxy[(5-methyl-3-phenyl-1,2-oxazole-4-carbonyl)amino]methyl}-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
oxacillin + H2O
-
 Bacillus cereus (2R,4S)-2-{(R)-carboxy[(5-methyl-3-phenyl-1,2-oxazole-4-carbonyl)amino]methyl}-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
oxacillin + H2O
-
 Bacillus cereus 569/H/9 (2R,4S)-2-{(R)-carboxy[(5-methyl-3-phenyl-1,2-oxazole-4-carbonyl)amino]methyl}-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
oxacillin + H2O
-
 Staphylococcus aureus PC1 (2R,4S)-2-{(R)-carboxy[(5-methyl-3-phenyl-1,2-oxazole-4-carbonyl)amino]methyl}-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?

Synonyms

Synonyms Comment Organism
beta-lactamse A-D A,C and D enzymes utilize a nucleophilic serine at the active center which becomes acylated by substrate, B enzymes are metalloenzymes requiring Zn2+ for activity Staphylococcus aureus
beta-lactamse A-D A,C and D enzymes utilize a nucleophilic serine at the active center which becomes acylated by substrate, B enzymes are metalloenzymes requiring Zn2+ for activity Bacillus cereus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
8
-
 Oxacillin
-
 Staphylococcus aureus
135
-
 benzylpenicillin
-
 Staphylococcus aureus
145
-
 Methicillin
-
 Bacillus cereus
261
-
 Oxacillin
-
 Bacillus cereus
550
-
 ampicillin
-
 Staphylococcus aureus
1350
-
 benzylpenicillin
-
 Bacillus cereus
5400
-
 ampicillin
-
 Bacillus cereus