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Literature summary for 3.5.2.3 extracted from

  • Lee, M.; Chan, C.W.; Graham, S.C.; Christopherson, R.I.; Guss, J.M.; Maher, M.J.
    Structures of ligand-free and inhibitor complexes of dihydroorotase from Escherichia coli: implications for loop movement in inhibitor design (2007), J. Mol. Biol., 370, 812-825.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
crystallized without ligand and in the presence of the inhibitors 2-oxo-1,2,3,6-tetrahydropyrimidine-4,6-dicarboxylate and 5-fluoroorotate, hanging drop vapour diffusion method with 15-20% (w/v) polyethylene glycol 3350, 0.1 M MES (pH 6.0-6.5), 75 mM MgCl2, 150 mM KCl (with ligand) or 20-25% polyethylene glycol 3350, 0.1 M Na HEPES (pH 7) and 0.2 M NaF (without ligand) Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
2-oxo-1,2,3,6-tetrahydropyrimidine-4,6-dicarboxylate
-
Escherichia coli
5-Fluoroorotate
-
Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P05020
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-dihydroorotate + H2O
-
Escherichia coli N-carbamoyl-L-aspartate
-
r
N-carbamoyl-L-aspartate
-
Escherichia coli L-dihydroorotate + H2O
-
r

Subunits

Subunits Comment Organism
homodimer x-ray crystallography Escherichia coli

Synonyms

Synonyms Comment Organism
DHOase
-
Escherichia coli