Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain X7014 | Toxoplasma gondii |
expression in Escherichia coli mutant strain SØ1263 and strain X7014 | Toxoplasma gondii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.063 | - |
dihydroorotate | purified recombinant enzyme, at 37°C | Toxoplasma gondii | |
0.0643 | - |
L-dihydroorotate | purified recombinant protein | Toxoplasma gondii | |
0.323 | - |
N-carbamoyl-L-aspartate | purified recombinant protein | Toxoplasma gondii | |
0.323 | - |
N-(aminocarbonyl)-L-aspartic acid | purified recombinant enzyme, at 37°C | Toxoplasma gondii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | - |
Toxoplasma gondii | |
Zn2+ | contains 3.33 mol zinc per mol protein | Toxoplasma gondii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44200 | - |
estimated from sequence of cDNA | Toxoplasma gondii |
45600 | - |
gel filtration, laser light scattering | Toxoplasma gondii |
45600 | - |
gel filtration and laser light scattering | Toxoplasma gondii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | O04904 | - |
- |
Escherichia coli | P05020 | - |
- |
Homo sapiens | P27708 | - |
- |
Mesocricetus auratus | P08955 | - |
- |
Mycobacterium leprae | - |
- |
- |
Plasmodium berghei | A0A509AX79 | - |
- |
Saccharomyces cerevisiae | - |
- |
- |
Toxoplasma gondii | Q8MXA5 | strain RH lambda Zap II cDNA library, strain PC117 and 5TN | - |
Toxoplasma gondii | Q8MXA5 | strains RH, PC117, and 5TN | - |
Trypanosoma cruzi | O76138 | - |
- |
Ustilago maydis | P31301 | - |
- |
Purification (Comment) | Organism |
---|---|
DEAE-Sephacel column chromatography | Toxoplasma gondii |
recombinant protein purified to homogeneity | Toxoplasma gondii |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
tachyzoite | - |
Toxoplasma gondii | - |
tachyzoite | maintained by serial passage in the peritoneal cavity of CFW mice | Toxoplasma gondii | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
18.3 | - |
in the biosynthetic direction | Toxoplasma gondii |
18.33 | - |
purified recombinant enzyme | Toxoplasma gondii |
18.4 | - |
in the degradative direction | Toxoplasma gondii |
Storage Stability | Organism |
---|---|
-70°C, HEPES buffer, pH 7.4, 0.5% glycerol | Toxoplasma gondii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dihydroorotate + H2O | - |
Toxoplasma gondii | N-carbamoyl-L-aspartate | - |
r | |
L-dihydroorotate + H2O | - |
Toxoplasma gondii | N-carbamoyl-L-aspartate | - |
r | |
N-carbamoyl-L-aspartate | - |
Toxoplasma gondii | L-dihydroorotate + H2O | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 44200, SDS-PAGE | Toxoplasma gondii |
monomer | 1 * 45600, gel filtration and laser light scattering | Toxoplasma gondii |
Synonyms | Comment | Organism |
---|---|---|
DHOase | - |
Saccharomyces cerevisiae |
DHOase | - |
Mycobacterium leprae |
DHOase | - |
Homo sapiens |
DHOase | - |
Mesocricetus auratus |
DHOase | - |
Toxoplasma gondii |
DHOase | - |
Plasmodium berghei |
DHOase | - |
Ustilago maydis |
DHOase | - |
Arabidopsis thaliana |
DHOase | - |
Escherichia coli |
DHOase | - |
Trypanosoma cruzi |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | in the biosynthetic direction | Toxoplasma gondii |
5 | 7.8 | in the degradative direction | Toxoplasma gondii |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Toxoplasma gondii | isoelectric focusing | - |
5.72 |