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Literature summary for 3.5.2.3 extracted from
- Inhibitors designed for the active site of dihydroorotase (2005), Bioorg. Chem., 33, 470-483.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C221S/C263S/C265S/C268S | this quadruple mutant is relatively stable to oxidation and has kinetic parameters consistent with reported values for wild type DHO | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| (S)-1,2,3,6-tetrahydro-6-oxopyridine-2-carboxylic acid | competitive inhibitor versus dihydroorotate and thio-dihydroorotate | Escherichia coli |  |
| 4(R)-hydroxy-6-oxo-piperidine-2(S)-carboxylic acid | competitive inhibitor versus dihydroorotate and thio-dihydroorotate | Escherichia coli | |
| 4(S)-hydroxy-6-oxo-piperidine-2(S)-carboxylic acid | competitive inhibitor versus dihydroorotate and thio-dihydroorotate | Escherichia coli | |
| 4,6-dioxo-piperidine-2-(S)-carboxylic acid | most active competitive inhibitor versus dihydroorotate and thio-dihydroorotate | Escherichia coli | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-dihydroorotate + H2O | - |
Escherichia coli | N-carbamoyl-L-aspartate | - |
r | |
| thio-dihydroorotate + H2O | - |
Escherichia coli | ? | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DHO | - |
Escherichia coli |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.076 | - |
4,6-dioxo-piperidine-2-(S)-carboxylic acid | with dihydroorotate as substrate, pH. 7.0 | Escherichia coli | |
| 0.096 | - |
4,6-dioxo-piperidine-2-(S)-carboxylic acid | with thio-dihydroorotate as substrate, pH. 7.0 | Escherichia coli | |
| 0.12 | - |
4,6-dioxo-piperidine-2-(S)-carboxylic acid | with thio-dihydroorotate as substrate, pH. 8.0 | Escherichia coli | |
| 0.21 | - |
4,6-dioxo-piperidine-2-(S)-carboxylic acid | with dihydroorotate as substrate, pH. 8.0 | Escherichia coli | |
| 0.32 | - |
4,6-dioxo-piperidine-2-(S)-carboxylic acid | with thio-dihydroorotate as substrate, pH. 9.0 | Escherichia coli | |
| 0.44 | - |
4,6-dioxo-piperidine-2-(S)-carboxylic acid | with dihydroorotate as substrate, pH. 9.0 | Escherichia coli | |
| 1.6 | - |
4(R)-hydroxy-6-oxo-piperidine-2(S)-carboxylic acid | with thio-dihydroorotate as substrate, pH. 8.0 | Escherichia coli | |
| 2.3 | - |
(S)-1,2,3,6-tetrahydro-6-oxopyridine-2-carboxylic acid | with thio-dihydroorotate as substrate, pH. 8.0 | Escherichia coli | |
| 3 | - |
4(S)-hydroxy-6-oxo-piperidine-2(S)-carboxylic acid | with thio-dihydroorotate as substrate, pH. 8.0 | Escherichia coli | |
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