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Literature summary for 3.5.2.20 extracted from

  • Sommer, T.; Bjerregaard-Andersen, K.; Uribe, L.; Etzerodt, M.; Diezemann, G.; Gauss, J.; Cascella, M.; Morth, J.P.
    A fundamental catalytic difference between zinc and manganese dependent enzymes revealed in a bacterial isatin hydrolase (2018), Sci. Rep., 8, 13104 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.2.20

Cloned(Commentary)

Cloned (Comment) Organism
-
 Roseibium aggregatum
-
 Ralstonia solanacearum

Crystallization (Commentary)

Crystallization (Comment) Organism
crystallized at 22°C using a protein concentration of 15 mg/ml in 5 mM Bis-Tris pH 7.0, 100 mM NaCl, and 1.0 mM MnCl2. The enzyme is crystallised by vapour diffusion using sitting drops and by vapour diffusion using hanging drops Roseibium aggregatum
crystallized at 22°C using a protein concentration of 15 mg/ml in 5 mM Bis-Tris pH 7.0, 100 mM NaCl, and 1.0 mM MnCl2. The enzyme is crystallised by vapour diffusion using sitting drops and by vapour diffusion using hanging drops Ralstonia solanacearum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00967
-
 isatin pH and temperature not specified in the publication Ralstonia solanacearum
0.0157
-
 isatin pH and temperature not specified in the publication Roseibium aggregatum

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ highest hydrolytic activity measured in the presence of Mn2+. The highly conserved Mn2+ binding site is located at the bottom of the substrate-binding pocket. The Mn2+ is found in an octahedral complex coordinated by His69, His73, Asp75, and two water molecules. The Mn2+ binding sites includes Gln219 Roseibium aggregatum
Mn2+ the Mn2+ binding sites includes Gln219 Ralstonia solanacearum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
isatin + H2O Roseibium aggregatum
-
 isatinate
-
 ?
isatin + H2O Ralstonia solanacearum
-
 isatinate
-
 ?
isatin + H2O Roseibium aggregatum ATCC 25650
-
 isatinate
-
 ?

Organism

Organism UniProt Comment Textmining
Ralstonia solanacearum Q8XYC3
-
-
Roseibium aggregatum A0P0F0
-
-
Roseibium aggregatum ATCC 25650 A0P0F0
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Roseibium aggregatum
-
 Ralstonia solanacearum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isatin + H2O
-
 Roseibium aggregatum isatinate
-
 ?
isatin + H2O
-
 Ralstonia solanacearum isatinate
-
 ?
isatin + H2O
-
 Roseibium aggregatum ATCC 25650 isatinate
-
 ?

Synonyms

Synonyms Comment Organism
isatin hydrolase A
-
 Roseibium aggregatum
isatin hydrolase A
-
 Ralstonia solanacearum
LaIHA
-
 Roseibium aggregatum
RsIHA
-
 Ralstonia solanacearum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
80
-
 isatin pH and temperature not specified in the publication Roseibium aggregatum
88
-
 isatin pH and temperature not specified in the publication Ralstonia solanacearum