Literature summary for 3.5.2.2 extracted from

Jin, Y.; Li, Y.; Sun, W.; Fan, S.; Feng, X.; Wang, K.; He, W.; Yang, Z.
The whole-cell immobilization of D-hydantoinase-engineered Escherichia coli for D-CpHPG biosynthesis (2016), Electron. J. Biotechnol., 21, 43-48 .

No PubMed abstract available

Search for more literature for 3.5.2.2

Cloned(Commentary)

Cloned (Comment)	Organism
functional recombinant expression in Escherichia coli strain BL21(DE3)	Streptomyces sp.

Protein Variants

Protein Variants	Comment	Organism
additional information	whole-cell immobilization on calcium alginate of D-hydantoinase-engineered Escherichia coli strain BL21(DE3) for D-carbamoyl-4-hydroxyphenylglycine (D-CpHPG) biosynthesis. Whole-cell immobilization method involves addition of 3.0% w/v alginate, 1.5% w/v diatomite, 0.05% w/v CaCl2, and 1.0 mM MnCl2. The optimized diameter of immobilized beads for the whole-cell biosynthesis is 2.60 mm. The maximized production rates of D-CpHPG are up to 76%, and the immobilized beads can be reused for 12 batches. Method optimization and evaluation, overview	Streptomyces sp.

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
DL-4-hydroxyphenylhydantoin + H2O	Streptomyces sp.	-	D-carbamoyl-4-hydroxyphenylglycine	-	?

Organism

Organism	UniProt	Comment	Textmining
Streptomyces sp.	-	from a library screened out of a marine sediment sample gathered at a depth of ca. 40 m from Heishijiao Bay, Dalian, China in 2008	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
DL-4-hydroxyphenylhydantoin + H2O	-	Streptomyces sp.	D-carbamoyl-4-hydroxyphenylglycine	-	?

Synonyms

Synonyms	Comment	Organism
D-CpHPG	-	Streptomyces sp.
D-hydantoinase	-	Streptomyces sp.

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
40	-	recombinant enzyme	Streptomyces sp.

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	recombinant enzyme	Streptomyces sp.

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Release 2023.1 (January 2023)