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Literature summary for 3.5.2.2 extracted from

  • Engel, U.; Syldatk, C.; Rudat, J.
    Stereoselective hydrolysis of aryl-substituted dihydropyrimidines by hydantoinases (2011), Appl. Microbiol. Biotechnol., 94, 1221-1231.
    View publication on PubMed
Search for more literature for 3.5.2.2

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Arthrobacter crystallopoietes
expression in Escherichia coli Ochrobactrum sp. G21
expression in Escherichia coli Delftia sp. I24

Organism

Organism UniProt Comment Textmining
Arthrobacter crystallopoietes
-
-
-
Arthrobacter crystallopoietes DSM 20117
-
-
-
Delftia sp. I24
-
-
-
Delftia sp. I24 DSM 18833
-
-
-
Ochrobactrum sp. G21
-
-
-
Ochrobactrum sp. G21 DSM 18828
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DL-6-(4-chlorophenyl)-5,6-dihydrouracil + H2O
-
 Arthrobacter crystallopoietes 4-chloro-N-carbamoyl-beta-phenylalanine
-
 ?
DL-6-(4-chlorophenyl)-5,6-dihydrouracil + H2O
-
 Ochrobactrum sp. G21 4-chloro-N-carbamoyl-beta-phenylalanine
-
 ?
DL-6-(4-chlorophenyl)-5,6-dihydrouracil + H2O
-
 Delftia sp. I24 4-chloro-N-carbamoyl-beta-phenylalanine
-
 ?
DL-6-(4-chlorophenyl)-5,6-dihydrouracil + H2O
-
 Ochrobactrum sp. G21 DSM 18828 4-chloro-N-carbamoyl-beta-phenylalanine
-
 ?
DL-6-(4-chlorophenyl)-5,6-dihydrouracil + H2O
-
 Arthrobacter crystallopoietes DSM 20117 4-chloro-N-carbamoyl-beta-phenylalanine
-
 ?
DL-6-(4-chlorophenyl)-5,6-dihydrouracil + H2O
-
 Delftia sp. I24 DSM 18833 4-chloro-N-carbamoyl-beta-phenylalanine
-
 ?
DL-6-phenyl-5,6-dihydrouracil + H2O
-
 Arthrobacter crystallopoietes N-rac-carbamoyl-beta-phenylalanine 27% conversion. Enzyme preferably hydrolyzes L-Phe-5,6-dihydrouracil to L-N-carbamoyl-beta-phenylalanine, 61% enantiomeric excess of L-enantiomer ?
DL-6-phenyl-5,6-dihydrouracil + H2O
-
 Ochrobactrum sp. G21 N-rac-carbamoyl-beta-phenylalanine enzyme does not display a significant entantioselectivity ?
DL-6-phenyl-5,6-dihydrouracil + H2O
-
 Delftia sp. I24 N-rac-carbamoyl-beta-phenylalanine enzyme does not display a significant entantioselectivity ?
DL-6-phenyl-5,6-dihydrouracil + H2O
-
 Ochrobactrum sp. G21 DSM 18828 N-rac-carbamoyl-beta-phenylalanine enzyme does not display a significant entantioselectivity ?
DL-6-phenyl-5,6-dihydrouracil + H2O
-
 Arthrobacter crystallopoietes DSM 20117 N-rac-carbamoyl-beta-phenylalanine 27% conversion. Enzyme preferably hydrolyzes L-Phe-5,6-dihydrouracil to L-N-carbamoyl-beta-phenylalanine, 61% enantiomeric excess of L-enantiomer ?
DL-6-phenyl-5,6-dihydrouracil + H2O
-
 Delftia sp. I24 DSM 18833 N-rac-carbamoyl-beta-phenylalanine enzyme does not display a significant entantioselectivity ?