Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain pE-HDT/BL21 | Pseudomonas putida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 97% residual activity at 1 mM | Pseudomonas putida | |
Cd2+ | 44% residual activity at 1 mM | Pseudomonas putida | |
Cu2+ | 0.4% residual activity at 1 mM | Pseudomonas putida | |
Zn2+ | 68% residual activity at 1 mM, the dissociation of purified enzyme into its subunits occurrs in the presence of 1 mM Zn2+ | Pseudomonas putida |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 79% increase of activity at 1 mM | Pseudomonas putida | |
Mg2+ | 20% increase of activity at 1 mM | Pseudomonas putida | |
Mn2+ | 74% increase of activity at 1 mM | Pseudomonas putida | |
Ni2+ | 54% increase of activity at 1 mM | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
2 * 52000, SDS-PAGE | Pseudomonas putida |
103000 | - |
SDS-PAGE | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | Q6TTR0 | - |
- |
Pseudomonas putida YZ-26 | Q6TTR0 | - |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate fractionation, phenyl-Sepharose column chromatography, and Sephacryl S-200 gel filtration | Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
10.65 | - |
after 3.4fold purification, using DL-5-phenylhydantoin as a substrate, in 50 mM Tris-HCl buffer, pH 8.0 | Pseudomonas putida |
11.84 | - |
after 3.4fold purification, using DL-p-hydroxyphenylhydantoin as a substrate, in 50 mM Tris-HCl buffer, pH 8.0 | Pseudomonas putida |
14.3 | - |
crude extract, using DL-hydantoin as a substrate, in 50 mM Tris-HCl buffer, pH 8.0 | Pseudomonas putida |
48.5 | - |
after 3.4fold purification, using DL-hydantoin as a substrate, in 50 mM Tris-HCl buffer, pH 8.0 | Pseudomonas putida |
600.8 | - |
after 3.4fold purification, using 5,6-dihydrouracil as a substrate, in 50 mM Tris-HCl buffer, pH 8.0 | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,6-dihydrouracil + H2O | - |
Pseudomonas putida | N-carbamoyl-beta-alanine | - |
? | |
5,6-dihydrouracil + H2O | - |
Pseudomonas putida YZ-26 | N-carbamoyl-beta-alanine | - |
? | |
DL-5-phenylhydantoin + H2O | - |
Pseudomonas putida | N-carbamoyl-D-phenylglycine | - |
? | |
DL-5-phenylhydantoin + H2O | - |
Pseudomonas putida YZ-26 | N-carbamoyl-D-phenylglycine | - |
? | |
DL-p-hydroxyphenylhydantoin + H2O | - |
Pseudomonas putida | N-carbamoyl-D-hydroxyphenylglycine | - |
? | |
DL-p-hydroxyphenylhydantoin + H2O | - |
Pseudomonas putida YZ-26 | N-carbamoyl-D-hydroxyphenylglycine | - |
? | |
hydantoin + H2O | - |
Pseudomonas putida | N-carbamoylglycine | - |
? | |
hydantoin + H2O | - |
Pseudomonas putida YZ-26 | N-carbamoylglycine | - |
? | |
additional information | no activity towards uracil and succinimide | Pseudomonas putida | ? | - |
? | |
additional information | no activity towards uracil and succinimide | Pseudomonas putida YZ-26 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 52000, SDS-PAGE | Pseudomonas putida |
Synonyms | Comment | Organism |
---|---|---|
D-hydantoinase | - |
Pseudomonas putida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Pseudomonas putida |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 55 | only stable at a temperature below 50°C, activity is rapidly lost over 55°C | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
- |
Pseudomonas putida |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 12 | the enzymatic activity is completely lost under pH 6.0, it maintains nearly 60% activity at a pH above 12 | Pseudomonas putida |