Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Agrobacterium tumefaciens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
L-5-ethylhydantoin | at 20°C | Agrobacterium tumefaciens | |
0.23 | - |
L-5-ethylhydantoin | at 25°C | Agrobacterium tumefaciens | |
0.4 | - |
D-5-methylthioethylhydantoin | at 20°C | Agrobacterium tumefaciens | |
0.4 | - |
L-5-methylthioethylhydantoin | at 20°C | Agrobacterium tumefaciens | |
0.43 | - |
L-5-ethylhydantoin | at 30°C | Agrobacterium tumefaciens | |
0.9 | - |
L-5-methylthioethylhydantoin | at 25°C | Agrobacterium tumefaciens | |
1.2 | - |
L-5-methylthioethylhydantoin | at 30°C | Agrobacterium tumefaciens | |
1.4 | - |
D-5-methylthioethylhydantoin | at 25°C | Agrobacterium tumefaciens | |
2.5 | - |
D-5-methylthioethylhydantoin | at 30°C | Agrobacterium tumefaciens | |
2.6 | - |
L-5-methylthioethylhydantoin | at 35°C | Agrobacterium tumefaciens | |
2.6 | - |
L-5-ethylhydantoin | at 35°C | Agrobacterium tumefaciens | |
3.4 | - |
D-5-ethylhydantoin | at 20°C | Agrobacterium tumefaciens | |
3.7 | - |
L-5-ethylhydantoin | at 40°C | Agrobacterium tumefaciens | |
4.7 | - |
L-5-methylthioethylhydantoin | at 40°C | Agrobacterium tumefaciens | |
5.1 | - |
D-5-ethylhydantoin | at 25°C | Agrobacterium tumefaciens | |
5.5 | - |
D-5-methylthioethylhydantoin | at 35°C | Agrobacterium tumefaciens | |
7 | - |
D-5-methylthioethylhydantoin | at 40°C | Agrobacterium tumefaciens | |
7.9 | - |
D-5-ethylhydantoin | at 30°C | Agrobacterium tumefaciens | |
8.2 | - |
D-5-ethylhydantoin | at 35°C | Agrobacterium tumefaciens | |
11.6 | - |
D-5-ethylhydantoin | at 40°C | Agrobacterium tumefaciens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Agrobacterium tumefaciens | - |
- |
- |
Agrobacterium tumefaciens BQL9 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
TALON metal affinity resin column chromatography | Agrobacterium tumefaciens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-5-ethylhydantoin + H2O | - |
Agrobacterium tumefaciens | ? | - |
r | |
D-5-ethylhydantoin + H2O | - |
Agrobacterium tumefaciens BQL9 | ? | - |
r | |
D-5-methylthioethylhydantoin + H2O | - |
Agrobacterium tumefaciens | ? | - |
r | |
D-5-methylthioethylhydantoin + H2O | - |
Agrobacterium tumefaciens BQL9 | ? | - |
r | |
L-5-ethylhydantoin + H2O | - |
Agrobacterium tumefaciens | ? | - |
r | |
L-5-ethylhydantoin + H2O | - |
Agrobacterium tumefaciens BQL9 | ? | - |
r | |
L-5-methylthioethylhydantoin + H2O | - |
Agrobacterium tumefaciens | ? | - |
r | |
L-5-methylthioethylhydantoin + H2O | - |
Agrobacterium tumefaciens BQL9 | ? | - |
r |
Synonyms | Comment | Organism |
---|---|---|
D-hydantoinase | - |
Agrobacterium tumefaciens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0055 | - |
L-5-ethylhydantoin | at 20°C | Agrobacterium tumefaciens | |
0.02 | - |
L-5-ethylhydantoin | at 25°C | Agrobacterium tumefaciens | |
0.061 | - |
L-5-ethylhydantoin | at 30°C | Agrobacterium tumefaciens | |
0.22 | - |
L-5-ethylhydantoin | at 35°C | Agrobacterium tumefaciens | |
0.44 | - |
L-5-ethylhydantoin | at 40°C | Agrobacterium tumefaciens | |
0.6 | - |
L-5-methylthioethylhydantoin | at 20°C | Agrobacterium tumefaciens | |
1 | - |
L-5-methylthioethylhydantoin | at 25°C | Agrobacterium tumefaciens | |
3.9 | - |
L-5-methylthioethylhydantoin | at 30°C | Agrobacterium tumefaciens | |
4.9 | - |
D-5-methylthioethylhydantoin | at 20°C | Agrobacterium tumefaciens | |
6.4 | - |
D-5-methylthioethylhydantoin | at 25°C | Agrobacterium tumefaciens | |
7.7 | - |
L-5-methylthioethylhydantoin | at 35°C | Agrobacterium tumefaciens | |
11.4 | - |
L-5-methylthioethylhydantoin | at 40°C | Agrobacterium tumefaciens | |
12.6 | - |
D-5-methylthioethylhydantoin | at 30°C | Agrobacterium tumefaciens | |
16.6 | - |
D-5-ethylhydantoin | at 20°C | Agrobacterium tumefaciens | |
20.5 | - |
D-5-ethylhydantoin | at 25°C | Agrobacterium tumefaciens | |
24.9 | - |
D-5-ethylhydantoin | at 30°C | Agrobacterium tumefaciens | |
26 | - |
D-5-methylthioethylhydantoin | at 35°C | Agrobacterium tumefaciens | |
27.3 | - |
D-5-ethylhydantoin | at 35°C | Agrobacterium tumefaciens | |
37.3 | - |
D-5-ethylhydantoin | at 40°C | Agrobacterium tumefaciens | |
92.6 | - |
D-5-methylthioethylhydantoin | at 40°C | Agrobacterium tumefaciens |