Literature summary for 3.5.2.2 extracted from

Niu, L.; Zhang, X.; Shi, Y.; Yuan, J.
Subunit dissociation and stability alteration of D-hydantoinase deleted at the terminal amino acid residue (2007), Biotechnol. Lett., 29, 303-308.

Search for more literature for 3.5.2.2

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21 (DE3) cells	Pseudomonas putida

Protein Variants

Protein Variants	Comment	Organism
additional information	HYDc1 with the C-terminal Arg deletion retains 43% activity, while HYDn1 with the N-terminal Ser deletion has no activity using DL-hydantoin as substrate	Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
54000	-	2 * 54000, SDS-PAGE	Pseudomonas putida
108000	-	SDS-PAGE	Pseudomonas putida

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas putida	Q6TTR0	YZ-26	-
Pseudomonas putida YZ-26	Q6TTR0	YZ-26	-

Purification (Commentary)

Purification (Comment)	Organism
ammonium sulfate precipitation, phenyl-Sepharose column chromatography, and Sephacryl S-200 gel filtration	Pseudomonas putida

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.9	-	crude extract	Pseudomonas putida
3.1	-	after 3.4fold purification	Pseudomonas putida

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
hydantoin + H2O	-	Pseudomonas putida	N-carbamoylglycine	-	?
hydantoin + H2O	-	Pseudomonas putida YZ-26	N-carbamoylglycine	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 54000, SDS-PAGE	Pseudomonas putida

Synonyms

Synonyms	Comment	Organism
D-hydantoinase	-	Pseudomonas putida
HYD	-	Pseudomonas putida

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
7	10	-	Pseudomonas putida

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Release 2023.1 (January 2023)