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Literature summary for 3.5.2.2 extracted from

  • Martinez-Rodriguez, S.; Gonzalez-Ramirez, L.A.; Clemente-Jimenez, J.M.; Rodriguez-Vico, F.; Las Heras-Vazquez, F.J.; Gavira, J.A.; Garcia-Ruiz, J.M.
    Crystallization and preliminary crystallographic studies of the recombinant dihydropyrimidinase from Sinorhizobium meliloti CECT4114 (2006), Acta Crystallogr. Sect. F, 62, 1223-1226.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.2.2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 cells Sinorhizobium meliloti

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapour diffusion method with 4.0 M sodium formate, 0.1 M sodium acetate pH 4.6 as precipitant in the presence of agarose at 0.1% (w/v) Sinorhizobium meliloti

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
200000
-
 gel filtration Sinorhizobium meliloti

Organism

Organism UniProt Comment Textmining
Sinorhizobium meliloti Q0PQZ5
-
-
Sinorhizobium meliloti CECT4114 Q0PQZ5
-
-

Purification (Commentary)

Purification (Comment) Organism
Talon metalaffinity resin column chromatography and Superdex 200 gel filtration Sinorhizobium meliloti

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,6-dihydrothymine + H2O
-
 Sinorhizobium meliloti ?
-
 ?
5,6-dihydrothymine + H2O
-
 Sinorhizobium meliloti CECT4114 ?
-
 ?
5,6-dihydrouracil + H2O
-
 Sinorhizobium meliloti 3-ureidopropionate
-
 ?
5,6-dihydrouracil + H2O
-
 Sinorhizobium meliloti CECT4114 3-ureidopropionate
-
 ?
DL-5-isopropylhydantoin + H2O
-
 Sinorhizobium meliloti N-carbamoyl-D-valine
-
 ?
DL-5-isopropylhydantoin + H2O
-
 Sinorhizobium meliloti CECT4114 N-carbamoyl-D-valine
-
 ?

Subunits

Subunits Comment Organism
tetramer x-ray crystallography Sinorhizobium meliloti

Synonyms

Synonyms Comment Organism
SmelDhp possesses both hydantoinase and dihydropyrimidinase activities Sinorhizobium meliloti