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Literature summary for 3.5.2.2 extracted from

  • Lin, L.L.; Hsu, W.H.; Hsu, W.Y.; Kan, S.C.; Hu, H.Y.
    Phylogenetic analysis and biochemical characterization of a thermostable dihydropyrimidinase from alkaliphilic Bacillus sp. TS-23 (2005), Antonie van Leeuwenhoek, 88, 189-197.
    View publication on PubMed
Search for more literature for 3.5.2.2

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of His6-tagged DHP in Escherichia coli Novablue cells Bacillus sp. (in: Bacteria)

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4
-
 dihydrouracil
-
 Bacillus sp. (in: Bacteria)
5
-
 hydantoin
-
 Bacillus sp. (in: Bacteria)

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ high stimulation, stimulates enzyme activity most effectively Bacillus sp. (in: Bacteria)
Cu2+ low stimulation Bacillus sp. (in: Bacteria)
Mn2+ high stimulation Bacillus sp. (in: Bacteria)
additional information no stimulation with Mg2+, Ni2+, Ca2+ and Fe2+ Bacillus sp. (in: Bacteria)
Zn2+ low stimulation Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
 SDS-PAGE corresponding well with calculated mass of the affinity-tagged translational product of the dhp gene Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Agrobacterium tumefaciens Q44184
-
-
Bacillus sp. (in: Bacteria) Q56S49 TS-23
-
Bacillus sp. (in: Bacteria) TS-23 Q56S49 TS-23
-
Geobacillus stearothermophilus Q45515
-
-
Pseudomonas putida Q59699
-
-
Ralstonia pickettii Q8VTT5
-
-
Streptomyces coelicolor O69809
-
-
Thermus sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by immobilized metal affinity chromatography Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.17
-
 EDTA-dialyzed/Co2+-substituted enzyme incubated with hydantoin as substrate Bacillus sp. (in: Bacteria)
0.36
-
 EDTA-dialyzed enzyme incubated with Fe2+ with DL-p-hydroxyphenylhydantoin as substrate Bacillus sp. (in: Bacteria)
0.46
-
 EDTA-dialyzed enzyme incubated with Ca2+ with DL-p-hydroxyphenylhydantoin as substrate Bacillus sp. (in: Bacteria)
0.49
-
 EDTA-dialyzed enzyme incubated with Ni2+ with DL-p-hydroxyphenylhydantoin as substrate Bacillus sp. (in: Bacteria)
0.52
-
 EDTA-dialyzed enzyme with D,L-p-hydroxyphenylhydantoin as substrate Bacillus sp. (in: Bacteria)
0.54
-
 EDTA-dialyzed enzyme incubated with Mg2+ with DL-p-hydroxyphenylhydantoin as substrate Bacillus sp. (in: Bacteria)
0.62
-
 EDTA-dialyzed/Co2+-substituted enzyme incubated with DL-5(2-(methylthio)-ethyl)hydantoin as substrate Bacillus sp. (in: Bacteria)
0.68
-
 EDTA-dialyzed enzyme incubated with Cu2+ with DL-p-hydroxyphenylhydantoin as substrate Bacillus sp. (in: Bacteria)
0.73
-
 EDTA-dialyzed enzyme incubated with Zn2+ with DL-p-hydroxyphenylhydantoin as substrate Bacillus sp. (in: Bacteria)
1.69
-
 EDTA-dialyzed/Co2+-substituted enzyme incubated with DL-p-hydroxyphenylhydantoin as substrate Bacillus sp. (in: Bacteria)
2.38
-
 EDTA-dialyzed enzyme incubated with Mn2+ with DL-p-hydroxyphenylhydantoin as substrate Bacillus sp. (in: Bacteria)
3.56
-
 EDTA-dialyzed/Co2+-substituted enzyme incubated with dihydrouracil as substrate Bacillus sp. (in: Bacteria)
3.6
-
 EDTA-dialyzed enzyme incubated with Co2+ with DL-p-hydroxyphenylhydantoin as substrate Bacillus sp. (in: Bacteria)

Storage Stability

Storage Stability Organism
50°C, 10 days Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,6-dihydrouracil + H2O highest activity of the EDTA-dialyzed/Co2+-substituted enzyme Bacillus sp. (in: Bacteria) ?
-
 ?
5,6-dihydrouracil + H2O highest activity of the EDTA-dialyzed/Co2+-substituted enzyme Bacillus sp. (in: Bacteria) TS-23 ?
-
 ?
DL-5(2-(methylthio)-ethyl)hydantoin + H2O EDTA-dialyzed/Co2+-substituted enzyme Bacillus sp. (in: Bacteria) ?
-
 ?
DL-5(2-(methylthio)-ethyl)hydantoin + H2O EDTA-dialyzed/Co2+-substituted enzyme Bacillus sp. (in: Bacteria) TS-23 ?
-
 ?
DL-p-hydroxyphenylhydantoin + H2O EDTA-dialyzed/Co2+-substituted enzyme Bacillus sp. (in: Bacteria) ?
-
 ?
DL-p-hydroxyphenylhydantoin + H2O EDTA-dialyzed/Co2+-substituted enzyme Bacillus sp. (in: Bacteria) TS-23 ?
-
 ?
hydantoin + H2O lowest activity of the EDTA-dialyzed/Co2+-substituted enzyme Bacillus sp. (in: Bacteria) ?
-
 ?
hydantoin + H2O lowest activity of the EDTA-dialyzed/Co2+-substituted enzyme Bacillus sp. (in: Bacteria) TS-23 ?
-
 ?

Synonyms

Synonyms Comment Organism
DHP
-
 Thermus sp.
DHP
-
 Salmonella enterica subsp. enterica serovar Typhimurium
DHP
-
 Brevibacillus agri
DHP
-
 Bacillus sp. (in: Bacteria)
DHP
-
 Geobacillus stearothermophilus
DHP
-
 Pseudomonas putida
DHP
-
 Ralstonia pickettii
DHP
-
 Agrobacterium tumefaciens
DHP
-
 Streptomyces coelicolor
DHP
-
 Oryza sativa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 DL-p-hydroxyphenylhydantoin as substrate Bacillus sp. (in: Bacteria)

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 80 DL-p-hydroxyphenylhydantoin as substrate Bacillus sp. (in: Bacteria)

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.04
-
 hydantoin
-
 Bacillus sp. (in: Bacteria)
6.2
-
 dihydrouracil
-
 Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 DL-p-hydroxyphenylhydantoin as substrate Bacillus sp. (in: Bacteria)

pH Range

pH Minimum pH Maximum Comment Organism
7 9 DL-p-hydroxyphenylhydantoin as substrate Bacillus sp. (in: Bacteria)