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Literature summary for 3.5.2.2 extracted from
- A D-specific hydantoin amidohydrolase: properties of the metalloenzyme purified from Arthrobacter crystallopoietes (1999), J. Mol. Catal. , B Enzym., 6, 387-397.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| freezing of the enzyme lead to its subsequent inactivation | Arthrobacter crystallopoietes |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 8-Hydroxyquinoline-5-sulfonic acid | - |
Arthrobacter crystallopoietes |  |
| 8-Hydroxyquinoline-5-sulfonic acid | - |
Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 7.9 | - |
DL-5-phenylhydantoin | - |
Arthrobacter crystallopoietes | |
| 8 | - |
phenylhydantoin | - |
Arthrobacter crystallopoietes | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | - |
Rattus norvegicus | |
| Zn2+ | - |
Paenarthrobacter aurescens | |
| Zn2+ | - |
Arthrobacter crystallopoietes | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60000 | - |
4 * 60000, SDS-PAGE | Arthrobacter crystallopoietes |
| 247000 | - |
calculated according to the repective amount of Zn2+ | Paenarthrobacter aurescens |
| 257000 | - |
gel filtration | Arthrobacter crystallopoietes |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,6-dihydrothymine + H2O | Rattus norvegicus | - |
? | - |
? | |
| 5,6-dihydrothymine + H2O | Pseudomonas putida | - |
? | - |
? | |
| 5,6-dihydrothymine + H2O | Bacillus sp. (in: Bacteria) | - |
? | - |
? | |
| 5,6-dihydrothymine + H2O | Arthrobacter crystallopoietes | - |
? | - |
? | |
| 5,6-dihydrothymine + H2O | Bacillus sp. (in: Bacteria) LU 1220 | - |
? | - |
? | |
| 5,6-dihydrouracil + H2O | Geobacillus stearothermophilus | - |
3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | Pseudomonas fluorescens | - |
3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | Pseudomonas putida | - |
3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | Bacillus sp. (in: Bacteria) | - |
3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | Agrobacterium tumefaciens | - |
3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | Paenarthrobacter aurescens | - |
3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | Arthrobacter crystallopoietes | - |
3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | Parageobacillus thermoglucosidasius | - |
3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | Bacillus sp. (in: Bacteria) LU 1220 | - |
3-ureidopropionate | - |
? | |
| hydantoin + H2O | Homo sapiens | - |
N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | Rattus norvegicus | - |
N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | Geobacillus stearothermophilus | - |
N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | Pseudomonas fluorescens | - |
N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | Pseudomonas sp. | - |
N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | Pseudomonas putida | - |
N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | Bacillus sp. (in: Bacteria) | - |
N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | Agrobacterium tumefaciens | - |
N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | Agrobacterium sp. | - |
N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | Arthrobacter crystallopoietes | - |
N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | Parageobacillus thermoglucosidasius | - |
N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | Pseudomonas sp. AJ-11220 | - |
N-carbamoyl glycine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Agrobacterium sp. | - |
- |
- |
| Agrobacterium sp. IP-I 671 | - |
- |
- |
| Agrobacterium tumefaciens | - |
- |
- |
| Arthrobacter crystallopoietes | - |
DSM 20117 | - |
| Bacillus sp. (in: Bacteria) | - |
LU 1220 | - |
| Bacillus sp. (in: Bacteria) LU 1220 | - |
LU 1220 | - |
| Geobacillus stearothermophilus | - |
- |
- |
| Homo sapiens | - |
human | - |
| Paenarthrobacter aurescens | - |
- |
- |
| Parageobacillus thermoglucosidasius | - |
- |
- |
| Pseudomonas fluorescens | - |
strain DSM 84 | - |
| Pseudomonas putida | - |
- |
- |
| Pseudomonas sp. | - |
- |
- |
| Pseudomonas sp. AJ-11220 | - |
- |
- |
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Arthrobacter crystallopoietes |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 100 | - |
- |
Arthrobacter crystallopoietes |
| 216 | - |
DL-5-phenylhydantoin as substrate | Arthrobacter crystallopoietes |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 5°C, phosphate buffer, can be stored under weakly alkaline conditions for at least several weeks, | Arthrobacter crystallopoietes |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,6-dihydrothymine + H2O | - |
Rattus norvegicus | ? | - |
? | |
| 5,6-dihydrothymine + H2O | - |
Pseudomonas putida | ? | - |
? | |
| 5,6-dihydrothymine + H2O | - |
Bacillus sp. (in: Bacteria) | ? | - |
? | |
| 5,6-dihydrothymine + H2O | - |
Agrobacterium sp. | ? | - |
? | |
| 5,6-dihydrothymine + H2O | - |
Arthrobacter crystallopoietes | ? | - |
? | |
| 5,6-dihydrothymine + H2O | - |
Agrobacterium sp. IP-I 671 | ? | - |
? | |
| 5,6-dihydrothymine + H2O | - |
Bacillus sp. (in: Bacteria) LU 1220 | ? | - |
? | |
| 5,6-dihydrouracil + H2O | - |
Rattus norvegicus | 3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | - |
Geobacillus stearothermophilus | 3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | - |
Pseudomonas fluorescens | 3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | - |
Pseudomonas putida | 3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | - |
Bacillus sp. (in: Bacteria) | 3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | - |
Agrobacterium tumefaciens | 3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | - |
Agrobacterium sp. | 3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | - |
Paenarthrobacter aurescens | 3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | - |
Arthrobacter crystallopoietes | 3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | - |
Parageobacillus thermoglucosidasius | 3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | - |
Agrobacterium sp. IP-I 671 | 3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | - |
Bacillus sp. (in: Bacteria) LU 1220 | 3-ureidopropionate | - |
? | |
| D-5-(2-methylthioethyl)hydantoin + H2O | - |
Geobacillus stearothermophilus | N-carbamoyl-D-methionine | - |
? | |
| D-5-(2-methylthioethyl)hydantoin + H2O | - |
Arthrobacter crystallopoietes | N-carbamoyl-D-methionine | - |
? | |
| DL-5-(2-methylthioethyl)-hydantoin + H2O | - |
Pseudomonas fluorescens | N-carbamoyl-D-methionine | - |
? | |
| DL-5-(2-methylthioethyl)-hydantoin + H2O | - |
Pseudomonas putida | N-carbamoyl-D-methionine | - |
? | |
| DL-5-(2-methylthioethyl)-hydantoin + H2O | - |
Bacillus sp. (in: Bacteria) | N-carbamoyl-D-methionine | - |
? | |
| DL-5-(2-methylthioethyl)-hydantoin + H2O | - |
Agrobacterium sp. | N-carbamoyl-D-methionine | - |
? | |
| DL-5-(2-methylthioethyl)-hydantoin + H2O | - |
Paenarthrobacter aurescens | N-carbamoyl-D-methionine | - |
? | |
| DL-5-(2-methylthioethyl)-hydantoin + H2O | strictly D-selective | Arthrobacter crystallopoietes | N-carbamoyl-D-methionine | - |
? | |
| DL-5-(2-methylthioethyl)-hydantoin + H2O | - |
Agrobacterium sp. IP-I 671 | N-carbamoyl-D-methionine | - |
? | |
| DL-5-(2-methylthioethyl)-hydantoin + H2O | - |
Bacillus sp. (in: Bacteria) LU 1220 | N-carbamoyl-D-methionine | - |
? | |
| DL-5-(p-hydroxyphenyl)hydantoin + H2O | - |
Geobacillus stearothermophilus | N-carbamoyl-p-hydroxyphenylglycine | - |
? | |
| DL-5-(p-hydroxyphenyl)hydantoin + H2O | - |
Pseudomonas putida | N-carbamoyl-p-hydroxyphenylglycine | - |
? | |
| DL-5-(p-hydroxyphenyl)hydantoin + H2O | - |
Bacillus sp. (in: Bacteria) | N-carbamoyl-p-hydroxyphenylglycine | - |
? | |
| DL-5-(p-hydroxyphenyl)hydantoin + H2O | - |
Agrobacterium sp. | N-carbamoyl-p-hydroxyphenylglycine | - |
? | |
| DL-5-(p-hydroxyphenyl)hydantoin + H2O | - |
Arthrobacter crystallopoietes | N-carbamoyl-p-hydroxyphenylglycine | - |
? | |
| DL-5-(p-hydroxyphenyl)hydantoin + H2O | - |
Agrobacterium sp. IP-I 671 | N-carbamoyl-p-hydroxyphenylglycine | - |
? | |
| DL-5-(p-hydroxyphenyl)hydantoin + H2O | - |
Bacillus sp. (in: Bacteria) LU 1220 | N-carbamoyl-p-hydroxyphenylglycine | - |
? | |
| DL-5-benzylhydantoin + H2O | - |
Pseudomonas fluorescens | ? | - |
? | |
| DL-5-benzylhydantoin + H2O | - |
Pseudomonas putida | ? | - |
? | |
| DL-5-benzylhydantoin + H2O | - |
Agrobacterium sp. | ? | - |
? | |
| DL-5-benzylhydantoin + H2O | - |
Arthrobacter crystallopoietes | ? | - |
? | |
| DL-5-benzylhydantoin + H2O | - |
Agrobacterium sp. IP-I 671 | ? | - |
? | |
| DL-5-indolylmethylhydantoin + H2O | - |
Pseudomonas fluorescens | L-tryptophan | - |
? | |
| DL-5-indolylmethylhydantoin + H2O | - |
Paenarthrobacter aurescens | L-tryptophan | - |
? | |
| DL-5-indolylmethylhydantoin + H2O | strictly D-selective | Arthrobacter crystallopoietes | L-tryptophan | - |
? | |
| DL-5-phenylhydantoin + H2O | - |
Rattus norvegicus | N-carbamoyl-D-phenylglycine | - |
? | |
| DL-5-phenylhydantoin + H2O | - |
Geobacillus stearothermophilus | N-carbamoyl-D-phenylglycine | - |
? | |
| DL-5-phenylhydantoin + H2O | - |
Pseudomonas putida | N-carbamoyl-D-phenylglycine | - |
? | |
| DL-5-phenylhydantoin + H2O | - |
Paenarthrobacter aurescens | N-carbamoyl-D-phenylglycine | - |
? | |
| DL-5-phenylhydantoin + H2O | - |
Arthrobacter crystallopoietes | N-carbamoyl-D-phenylglycine | - |
? | |
| DL-5-thienylmethylhydantoin + H2O | - |
Arthrobacter crystallopoietes | ? | - |
? | |
| hydantoin + H2O | - |
Geobacillus stearothermophilus | hydantoic acid | - |
? | |
| hydantoin + H2O | - |
Pseudomonas fluorescens | hydantoic acid | - |
? | |
| hydantoin + H2O | - |
Bacillus sp. (in: Bacteria) | hydantoic acid | - |
? | |
| hydantoin + H2O | - |
Parageobacillus thermoglucosidasius | hydantoic acid | - |
? | |
| hydantoin + H2O | - |
Bacillus sp. (in: Bacteria) LU 1220 | hydantoic acid | - |
? | |
| hydantoin + H2O | - |
Homo sapiens | N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | - |
Rattus norvegicus | N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | - |
Geobacillus stearothermophilus | N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | - |
Pseudomonas fluorescens | N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | - |
Pseudomonas sp. | N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | - |
Pseudomonas putida | N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | - |
Bacillus sp. (in: Bacteria) | N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | - |
Agrobacterium tumefaciens | N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | - |
Agrobacterium sp. | N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | - |
Arthrobacter crystallopoietes | N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | - |
Parageobacillus thermoglucosidasius | N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | - |
Pseudomonas sp. AJ-11220 | N-carbamoyl glycine | - |
? | |
| additional information | DL-5-phenylhydantoin is no substrate | Paenarthrobacter aurescens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | - |
Rattus norvegicus |
| tetramer | - |
Geobacillus stearothermophilus |
| tetramer | - |
Pseudomonas fluorescens |
| tetramer | - |
Paenarthrobacter aurescens |
| tetramer | 4 * 60000, SDS-PAGE | Arthrobacter crystallopoietes |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | 60 | - |
Arthrobacter crystallopoietes |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Agrobacterium sp. |
| 50 | - |
50% initial activity remains after 6 h, activity decreases within min by incubating at 60°C | Arthrobacter crystallopoietes |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Geobacillus stearothermophilus |
| 8 | - |
- |
Pseudomonas fluorescens |
| 8 | - |
- |
Arthrobacter crystallopoietes |
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