Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Uracil | induces biosynthesis of enzyme | Geobacillus thermocatenulatus |
Application | Comment | Organism |
---|---|---|
synthesis | commercial production of optically pure amino acids for synthesis of antibiotics, pharmaceuticals, artificial sweeteners and biologically active peptides, food ingredients, pyrethroids, pesticides, antimicrobial and antiviral agents and other agrochemicals | Geobacillus stearothermophilus |
synthesis | commercial production of optically pure amino acids for synthesis of antibiotics, pharmaceuticals, artificial sweeteners and biologically active peptides, food ingredients, pyrethroids, pesticides, antimicrobial and antiviral agents and other agrochemicals | Geobacillus thermocatenulatus |
synthesis | thermostable enzyme with great biotechnological potential | Geobacillus thermocatenulatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
30.7 | - |
p-hydroxyphenylhydantoin | - |
Geobacillus thermocatenulatus | |
47.2 | - |
p-hydroxyphenylhydantoin | - |
Geobacillus stearothermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | - |
Geobacillus stearothermophilus | |
Co2+ | - |
Geobacillus thermocatenulatus | |
Fe2+ | - |
Geobacillus thermocatenulatus | |
Mn2+ | - |
Geobacillus stearothermophilus | |
Mn2+ | - |
Geobacillus thermocatenulatus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56000 | - |
4 * 56000, SDS-PAGE | Geobacillus thermocatenulatus |
120000 | - |
strain SD-1 | Geobacillus stearothermophilus |
200000 | - |
strain NS1122A | Geobacillus stearothermophilus |
230000 | - |
gel filtration | Geobacillus thermocatenulatus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
hydantoin + H2O | Geobacillus stearothermophilus | - |
N-carbamoyl glycine | - |
? | |
hydantoin + H2O | Pseudomonas sp. | - |
N-carbamoyl glycine | - |
? | |
hydantoin + H2O | Agrobacterium sp. | - |
N-carbamoyl glycine | - |
? | |
hydantoin + H2O | Geobacillus thermocatenulatus | - |
N-carbamoyl glycine | - |
? | |
hydantoin + H2O | Geobacillus stearothermophilus SD-1 | - |
N-carbamoyl glycine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Agrobacterium sp. | - |
- |
- |
Geobacillus stearothermophilus | - |
- |
- |
Geobacillus stearothermophilus | - |
strains NS1122A and LU1220 | - |
Geobacillus stearothermophilus SD-1 | - |
- |
- |
Geobacillus thermocatenulatus | - |
- |
- |
Geobacillus thermocatenulatus GH-2 | - |
- |
- |
Pseudomonas sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Geobacillus thermocatenulatus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
10.9 | - |
isopropylhydantoin as substrate | Geobacillus thermocatenulatus |
27.5 | - |
dihydrouracil as substrate | Geobacillus thermocatenulatus |
91.6 | - |
hydroxyphenylhydantoin as substrate | Geobacillus thermocatenulatus |
101.5 | - |
hydantoin as substrate | Geobacillus thermocatenulatus |
236.9 | - |
phenylhydantoin as substrate | Geobacillus thermocatenulatus |
461 | - |
- |
Geobacillus thermocatenulatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,6-dihydrouracil + H2O | - |
Geobacillus stearothermophilus | 3-ureidopropionate | - |
? | |
5,6-dihydrouracil + H2O | - |
Agrobacterium sp. | 3-ureidopropionate | - |
? | |
5,6-dihydrouracil + H2O | - |
Geobacillus thermocatenulatus | 3-ureidopropionate | - |
? | |
5,6-dihydrouracil + H2O | - |
Geobacillus stearothermophilus SD-1 | 3-ureidopropionate | - |
? | |
5,6-dihydrouracil + H2O | - |
Geobacillus thermocatenulatus GH-2 | 3-ureidopropionate | - |
? | |
DL-5-(2-methylthioethyl)-hydantoin + H2O | - |
Agrobacterium sp. | N-carbamoyl-D-methionine | - |
? | |
DL-5-(p-hydroxyphenyl)hydantoin + H2O | - |
Geobacillus stearothermophilus | N-carbamoyl-p-hydroxyphenylglycine | - |
? | |
DL-5-(p-hydroxyphenyl)hydantoin + H2O | - |
Agrobacterium sp. | N-carbamoyl-p-hydroxyphenylglycine | - |
? | |
DL-5-(p-hydroxyphenyl)hydantoin + H2O | - |
Geobacillus thermocatenulatus | N-carbamoyl-p-hydroxyphenylglycine | - |
? | |
DL-5-(p-hydroxyphenyl)hydantoin + H2O | - |
Geobacillus stearothermophilus SD-1 | N-carbamoyl-p-hydroxyphenylglycine | - |
? | |
DL-5-(p-hydroxyphenyl)hydantoin + H2O | - |
Geobacillus thermocatenulatus GH-2 | N-carbamoyl-p-hydroxyphenylglycine | - |
? | |
DL-5-isobutyl-hydantoin + H2O | - |
Agrobacterium sp. | ? | - |
? | |
DL-5-isopropylhydantoin + H2O | - |
Agrobacterium sp. | N-carbamoyl-D-valine | - |
? | |
DL-5-isopropylhydantoin + H2O | - |
Geobacillus thermocatenulatus | N-carbamoyl-D-valine | - |
? | |
DL-5-isopropylhydantoin + H2O | - |
Geobacillus thermocatenulatus GH-2 | N-carbamoyl-D-valine | - |
? | |
DL-5-phenylhydantoin + H2O | - |
Geobacillus stearothermophilus | N-carbamoyl-D-phenylglycine | - |
? | |
DL-5-phenylhydantoin + H2O | - |
Geobacillus thermocatenulatus | N-carbamoyl-D-phenylglycine | - |
? | |
DL-5-phenylhydantoin + H2O | - |
Geobacillus stearothermophilus SD-1 | N-carbamoyl-D-phenylglycine | - |
? | |
DL-5-phenylhydantoin + H2O | - |
Geobacillus thermocatenulatus GH-2 | N-carbamoyl-D-phenylglycine | - |
? | |
hydantoin + H2O | - |
Geobacillus stearothermophilus | hydantoic acid | - |
? | |
hydantoin + H2O | - |
Geobacillus thermocatenulatus | hydantoic acid | - |
? | |
hydantoin + H2O | - |
Geobacillus stearothermophilus SD-1 | hydantoic acid | - |
? | |
hydantoin + H2O | - |
Geobacillus thermocatenulatus GH-2 | hydantoic acid | - |
? | |
hydantoin + H2O | - |
Geobacillus stearothermophilus | N-carbamoyl glycine | - |
? | |
hydantoin + H2O | - |
Pseudomonas sp. | N-carbamoyl glycine | - |
? | |
hydantoin + H2O | - |
Agrobacterium sp. | N-carbamoyl glycine | - |
? | |
hydantoin + H2O | - |
Geobacillus thermocatenulatus | N-carbamoyl glycine | - |
? | |
hydantoin + H2O | - |
Geobacillus stearothermophilus SD-1 | N-carbamoyl glycine | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | - |
Geobacillus stearothermophilus |
tetramer | 4 * 56000, SDS-PAGE | Geobacillus thermocatenulatus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Geobacillus stearothermophilus |
65 | - |
- |
Geobacillus thermocatenulatus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 80 | - |
Geobacillus thermocatenulatus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
- |
Geobacillus stearothermophilus |
80 | - |
half-life about 45 min, stable up to 75°C | Geobacillus thermocatenulatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Geobacillus thermocatenulatus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 9.5 | - |
Geobacillus thermocatenulatus |