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Literature summary for 3.5.2.2 extracted from
- Substrate-dependent enantioselectivity of a novel hydantoinase from Arthrobacter aurescens DSM 3745: Purification and characterization as new member of cyclic amidases (1998), J. Biotechnol., 61, 1-13.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | - |
Agrobacterium tumefaciens |
| synthesis | production of enantiomeric pure nonproteinogenic amino acids, currently under investigation in bioprocess scale for industrial application | Paenarthrobacter aurescens |
| synthesis | commercial production of optically pure amino acids for synthesis of antibiotics, pharmaceuticals, artificial sweeteners and biologically active peptides, food ingredients, pyrethroids, pesticides, antimicrobial and antiviral agents and other agrochemicals | Geobacillus stearothermophilus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| D-hydantoinase gene cloned, sequenced and expressed in Escherichia coli | Pseudomonas putida |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Pseudomonas putida |
- |
Paenarthrobacter aurescens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 28 | - |
DL-5-(2-methylthioethyl)-hydantoin | - |
Bos taurus |  |
| 28 | - |
DL-5-(2-methylthioethyl)-hydantoin | - |
Pseudomonas putida | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 49670 | - |
4 * 49670 | Paenarthrobacter aurescens |
| 49680 | - |
MALDI-MS studies, single charged monomer | Paenarthrobacter aurescens |
| 53000 | - |
SDS-PAGE | Paenarthrobacter aurescens |
| 200000 | - |
- |
Geobacillus stearothermophilus |
| 200000 | - |
native enzyme | Paenarthrobacter aurescens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,6-dihydrouracil + H2O | Bos taurus | - |
3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | Geobacillus stearothermophilus | - |
3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | Pseudomonas putida | - |
3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | Agrobacterium tumefaciens | - |
3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | Paenarthrobacter aurescens | - |
3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | Geobacillus stearothermophilus JC2310 | - |
3-ureidopropionate | - |
? | |
| dihydrothymidine + H2O | Paenarthrobacter aurescens | - |
? | - |
? | |
| hydantoin + H2O | Brevibacillus brevis | - |
N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | Rattus norvegicus | - |
N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | Bos taurus | - |
N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | Geobacillus stearothermophilus | - |
N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | Pseudomonas putida | - |
N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | Pseudomonas stutzeri | - |
N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | Arthrobacter sp. | - |
N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | Agrobacterium tumefaciens | - |
N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | Flavobacterium sp. | - |
N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | Geobacillus stearothermophilus JC2310 | - |
N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | Arthrobacter sp. DSM 3747 | - |
N-carbamoyl glycine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Agrobacterium tumefaciens | - |
- |
- |
| Arthrobacter sp. | - |
DSM3747 | - |
| Arthrobacter sp. DSM 3747 | - |
DSM3747 | - |
| Bos taurus | - |
calf | - |
| Bos taurus | - |
bovine | - |
| Brevibacillus brevis | - |
- |
- |
| Flavobacterium sp. | - |
- |
- |
| Geobacillus stearothermophilus | - |
- |
- |
| Geobacillus stearothermophilus JC2310 | - |
- |
- |
| Paenarthrobacter aurescens | - |
DSM 3745 | - |
| Pseudomonas putida | - |
- |
- |
| Pseudomonas stutzeri | - |
- |
- |
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas putida |
- |
Paenarthrobacter aurescens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
| liver | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.1 | - |
10 mM dihydrouracil as substrate | Paenarthrobacter aurescens |
| 0.3 | - |
DL-phenylhydantoin, 50 mM | Paenarthrobacter aurescens |
| 0.4 | - |
10 mM dihydrothymine as substrate | Paenarthrobacter aurescens |
| 0.6 | - |
DL-methylhydantoin, 57 mM | Paenarthrobacter aurescens |
| 1.5 | - |
L-methylthioethylhydantoin, 2 mM | Paenarthrobacter aurescens |
| 5.4 | - |
D-methylthioethylhydantoin, 2 mM | Paenarthrobacter aurescens |
| 12 | - |
L-methylthioethylhydantoin, 20 mM | Paenarthrobacter aurescens |
| 13 | - |
DL-5-indolylmethylhydantoin as substrate | Paenarthrobacter aurescens |
| 30 | - |
DL-methylthioethylhydantoin, 40 mM | Paenarthrobacter aurescens |
| 37 | - |
D-methylthioethylhydantoin, 20 mM | Paenarthrobacter aurescens |
| 70 | - |
L-5-indolylmethylhydantoin as substrate | Paenarthrobacter aurescens |
| 72 | - |
- |
Paenarthrobacter aurescens |
| 76.6 | - |
DL-fluorobromylhydantoin as substrate | Paenarthrobacter aurescens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,6-dihydrouracil + H2O | - |
Bos taurus | 3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | - |
Geobacillus stearothermophilus | 3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | - |
Pseudomonas putida | 3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | - |
Agrobacterium tumefaciens | 3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | - |
Paenarthrobacter aurescens | 3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | - |
Geobacillus stearothermophilus JC2310 | 3-ureidopropionate | - |
? | |
| dihydrothymidine + H2O | - |
Paenarthrobacter aurescens | ? | - |
? | |
| DL-5-(2-methylthioethyl)-hydantoin + H2O | - |
Bos taurus | N-carbamoyl-D-methionine | - |
? | |
| DL-5-(2-methylthioethyl)-hydantoin + H2O | - |
Pseudomonas putida | N-carbamoyl-D-methionine | - |
? | |
| DL-5-(2-methylthioethyl)-hydantoin + H2O | - |
Paenarthrobacter aurescens | N-carbamoyl-D-methionine | - |
? | |
| DL-5-indolylmethylhydantoin + H2O | - |
Flavobacterium sp. | L-tryptophan | - |
? | |
| DL-5-indolylmethylhydantoin + H2O | strictly L-selective for the cleavage | Paenarthrobacter aurescens | L-tryptophan | - |
? | |
| DL-5-phenylhydantoin + H2O | - |
Rattus norvegicus | N-carbamoyl-D-phenylglycine | - |
? | |
| DL-5-phenylhydantoin + H2O | - |
Bos taurus | N-carbamoyl-D-phenylglycine | - |
? | |
| DL-5-phenylhydantoin + H2O | - |
Geobacillus stearothermophilus | N-carbamoyl-D-phenylglycine | - |
? | |
| DL-5-phenylhydantoin + H2O | - |
Pseudomonas putida | N-carbamoyl-D-phenylglycine | - |
? | |
| DL-5-phenylhydantoin + H2O | - |
Flavobacterium sp. | N-carbamoyl-D-phenylglycine | - |
? | |
| DL-5-phenylhydantoin + H2O | - |
Paenarthrobacter aurescens | N-carbamoyl-D-phenylglycine | - |
? | |
| DL-5-phenylhydantoin + H2O | - |
Geobacillus stearothermophilus JC2310 | N-carbamoyl-D-phenylglycine | - |
? | |
| DL-fluorobromylhydantoin + H2O | - |
Paenarthrobacter aurescens | ? | - |
? | |
| DL-methylthioethylhydantoin + H2O | D-selective for the hydrolysis | Paenarthrobacter aurescens | ? | - |
? | |
| hydantoin + H2O | - |
Bos taurus | hydantoic acid | - |
? | |
| hydantoin + H2O | - |
Geobacillus stearothermophilus | hydantoic acid | - |
? | |
| hydantoin + H2O | - |
Pseudomonas stutzeri | hydantoic acid | - |
? | |
| hydantoin + H2O | - |
Geobacillus stearothermophilus JC2310 | hydantoic acid | - |
? | |
| hydantoin + H2O | - |
Brevibacillus brevis | N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | - |
Rattus norvegicus | N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | - |
Bos taurus | N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | - |
Geobacillus stearothermophilus | N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | - |
Pseudomonas putida | N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | - |
Pseudomonas stutzeri | N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | - |
Arthrobacter sp. | N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | - |
Agrobacterium tumefaciens | N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | - |
Flavobacterium sp. | N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | - |
Geobacillus stearothermophilus JC2310 | N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | - |
Arthrobacter sp. DSM 3747 | N-carbamoyl glycine | - |
? | |
| additional information | - |
Rattus norvegicus | ? | - |
? | |
| additional information | - |
Bos taurus | ? | - |
? | |
| additional information | - |
Pseudomonas putida | ? | - |
? | |
| additional information | hydantoin, urea, allantoin and dihydroorotate are no substrates | Paenarthrobacter aurescens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | - |
Rattus norvegicus |
| tetramer | - |
Bos taurus |
| tetramer | - |
Geobacillus stearothermophilus |
| tetramer | - |
Arthrobacter sp. |
| tetramer | 4 * 49670 | Paenarthrobacter aurescens |
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