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Literature summary for 3.5.2.2 extracted from
- Properties of D-hydantoinase from Agrobacterium tumefaciens and its use for the preparation of N-carabmyl D-amino acids (1995), Biochem. Biophys. Res. Commun., 216, 1095-1100.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | - |
Agrobacterium tumefaciens |
| synthesis | commercial production of optically pure amino acids for synthesis of antibiotics, pharmaceuticals, artificial sweeteners and biologically active peptides, food ingredients, pyrethroids, pesticides, antimicrobial and antiviral agents and other agrochemicals | Agrobacterium tumefaciens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,6-dihydrouracil + H2O | Agrobacterium tumefaciens | - |
3-ureidopropionate | - |
? |  |
| 5,6-dihydrouracil + H2O | Agrobacterium sp. | purified preparation demonstrates no activity on this substrate | 3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | Agrobacterium sp. IP-I 671 | purified preparation demonstrates no activity on this substrate | 3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | Agrobacterium tumefaciens 47C | - |
3-ureidopropionate | - |
? | |
| hydantoin + H2O | Agrobacterium tumefaciens | - |
N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | Agrobacterium sp. | - |
N-carbamoyl glycine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Agrobacterium sp. | - |
- |
- |
| Agrobacterium sp. IP-I 671 | - |
- |
- |
| Agrobacterium tumefaciens | - |
- |
- |
| Agrobacterium tumefaciens 47C | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.44 | - |
DL-hydantoin as substrate | Agrobacterium tumefaciens |
| 1.67 | - |
DL-5-methylhydantoin as substrate | Agrobacterium tumefaciens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,6-dihydrouracil + H2O | - |
Agrobacterium tumefaciens | 3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | - |
Agrobacterium sp. | 3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | purified preparation demonstrates no activity on this substrate | Agrobacterium sp. | 3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | - |
Agrobacterium sp. IP-I 671 | 3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | purified preparation demonstrates no activity on this substrate | Agrobacterium sp. IP-I 671 | 3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | - |
Agrobacterium tumefaciens 47C | 3-ureidopropionate | - |
? | |
| 5-hydroxymethylhydantoin + H2O | - |
Agrobacterium tumefaciens | ? | - |
? | |
| DL-5-(2-methylthioethyl)-hydantoin + H2O | - |
Agrobacterium tumefaciens | N-carbamoyl-D-methionine | - |
? | |
| DL-5-(2-methylthioethyl)-hydantoin + H2O | - |
Agrobacterium sp. | N-carbamoyl-D-methionine | - |
? | |
| DL-5-benzylhydantoin + H2O | - |
Agrobacterium sp. | ? | - |
? | |
| DL-5-benzylhydantoin + H2O | only slight activity | Agrobacterium tumefaciens | ? | - |
? | |
| DL-5-isobutyl-hydantoin + H2O | - |
Agrobacterium tumefaciens | ? | - |
? | |
| DL-5-isobutyl-hydantoin + H2O | - |
Agrobacterium sp. | ? | - |
? | |
| DL-5-isobutyl-hydantoin + H2O | - |
Agrobacterium sp. IP-I 671 | ? | - |
? | |
| DL-5-isobutyl-hydantoin + H2O | - |
Agrobacterium tumefaciens 47C | ? | - |
? | |
| DL-5-isopropylhydantoin + H2O | - |
Agrobacterium tumefaciens | N-carbamoyl-D-valine | - |
? | |
| DL-5-isopropylhydantoin + H2O | - |
Agrobacterium sp. | N-carbamoyl-D-valine | - |
? | |
| DL-5-isopropylhydantoin + H2O | - |
Agrobacterium sp. IP-I 671 | N-carbamoyl-D-valine | - |
? | |
| DL-5-isopropylhydantoin + H2O | - |
Agrobacterium tumefaciens 47C | N-carbamoyl-D-valine | - |
? | |
| DL-5-methylhydantoin | - |
Agrobacterium tumefaciens | ? | - |
? | |
| DL-5-phenylhydantoin + H2O | - |
Agrobacterium tumefaciens | N-carbamoyl-D-phenylglycine | - |
? | |
| DL-5-sec-butylhydantoin + H2O | - |
Agrobacterium tumefaciens | ? | - |
? | |
| DL-5-sec-butylhydantoin + H2O | - |
Agrobacterium sp. | ? | - |
? | |
| DL-5-sec-butylhydantoin + H2O | - |
Agrobacterium sp. IP-I 671 | ? | - |
? | |
| DL-5-sec-butylhydantoin + H2O | - |
Agrobacterium tumefaciens 47C | ? | - |
? | |
| hydantoin + H2O | - |
Agrobacterium tumefaciens | N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | - |
Agrobacterium sp. | N-carbamoyl glycine | - |
? | |
| additional information | disubstituted hydantoins or 1-substituted hydantoins are no substrates | Agrobacterium tumefaciens | ? | - |
? | |
| additional information | disubstituted hydantoins or 1-substituted hydantoins are no substrates | Agrobacterium tumefaciens 47C | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Agrobacterium tumefaciens |
| 70 | - |
- |
Agrobacterium sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 10 | - |
- |
Agrobacterium tumefaciens |
| 10 | - |
- |
Agrobacterium sp. |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 11 | at 40°C | Agrobacterium tumefaciens |
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