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Literature summary for 3.5.2.2 extracted from
- Microbial conversion of DL-5-substituted hydantoins to the corresponding L-amino acids by Bacillus stearothermophilus NS1122A (1994), Biosci. Biotechnol. Biochem., 58, 265-270.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DL-hydantoinase cloned from 172-kb native plasmid | Pseudomonas sp. |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | CuSO4*5H2O strongly inhibits L-methionine production | Geobacillus stearothermophilus |  |
| Cu2+ | - |
Pseudomonas fluorescens | |
| Zn2+ | ZnSO4*7H2O strongly inhibits L-methionine production | Geobacillus stearothermophilus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | - |
Bos taurus | |
| Co2+ | stimulates production of L-methionine | Geobacillus stearothermophilus | |
| Mn2+ | - |
Bos taurus | |
| Mn2+ | - |
Thermus sp. | |
| Mn2+ | stimulates production of L-methionine | Geobacillus stearothermophilus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
4 * 55000, SDS-PAGE | Geobacillus stearothermophilus |
| 155000 | 200000 | - |
Arthrobacter sp. |
| 200000 | - |
gel filtration | Geobacillus stearothermophilus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| hydantoin + H2O | Bos taurus | - |
N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | Geobacillus stearothermophilus | - |
N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | Pseudomonas fluorescens | - |
N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | Pseudomonas sp. | - |
N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | Pseudomonas putida | - |
N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | Arthrobacter sp. | - |
N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | Thermus sp. | - |
N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | Flavobacterium sp. | - |
N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | Pseudomonas sp. NS671 | - |
N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | Arthrobacter sp. DSM 3747 | - |
N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | Flavobacterium sp. AJ-3912 | - |
N-carbamoyl glycine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arthrobacter sp. | - |
DSM3747 | - |
| Arthrobacter sp. DSM 3747 | - |
DSM3747 | - |
| Bos taurus | - |
bovine | - |
| Flavobacterium sp. | - |
AJ-3912 | - |
| Flavobacterium sp. AJ-3912 | - |
AJ-3912 | - |
| Geobacillus stearothermophilus | - |
strains NS1122A and LU1220 | - |
| Pseudomonas fluorescens | - |
- |
- |
| Pseudomonas putida | - |
- |
- |
| Pseudomonas sp. | - |
- |
- |
| Pseudomonas sp. NS671 | - |
- |
- |
| Thermus sp. | - |
thermophilic bacterial strains, CBS 303.80 and CBS363.80, asporogenic gram-negative | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Geobacillus stearothermophilus |
- |
Pseudomonas sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,6-dihydrouracil + H2O | - |
Bos taurus | 3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | - |
Geobacillus stearothermophilus | 3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | - |
Pseudomonas fluorescens | 3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | - |
Pseudomonas putida | 3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | - |
Thermus sp. | 3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | - |
Flavobacterium sp. | 3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | - |
Flavobacterium sp. AJ-3912 | 3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | - |
Geobacillus stearothermophilus NS1122A | 3-ureidopropionate | - |
? | |
| D-5-(1-methylpropyl)hydantoin + H2O | - |
Geobacillus stearothermophilus | N-carbamoyl-D-isoleucine | - |
? | |
| D-5-(2-methylthioethyl)hydantoin + H2O | - |
Geobacillus stearothermophilus | N-carbamoyl-D-methionine | - |
? | |
| D-5-benzylhydantoin + H2O | - |
Geobacillus stearothermophilus | N-carbamoyl-D-phenylalanine | - |
? | |
| D-5-isobutylhydantoin + H2O | - |
Geobacillus stearothermophilus | N-carbamoyl-D-leucine | - |
? | |
| D-5-isobutylhydantoin + H2O | - |
Geobacillus stearothermophilus NS1122A | N-carbamoyl-D-leucine | - |
? | |
| D-5-isopropylhydantoin + H2O | - |
Geobacillus stearothermophilus | N-carbamoyl-D-valine | - |
? | |
| D-5-isopropylhydantoin + H2O | - |
Geobacillus stearothermophilus NS1122A | N-carbamoyl-D-valine | - |
? | |
| D-5-methylhydantoin + H2O | - |
Bos taurus | N-carbamoyl-D-alanine | - |
? | |
| D-5-methylhydantoin + H2O | - |
Geobacillus stearothermophilus | N-carbamoyl-D-alanine | - |
? | |
| D-5-methylhydantoin + H2O | - |
Geobacillus stearothermophilus NS1122A | N-carbamoyl-D-alanine | - |
? | |
| hydantoin + H2O | - |
Bos taurus | N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | - |
Geobacillus stearothermophilus | N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | - |
Pseudomonas fluorescens | N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | - |
Pseudomonas sp. | N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | - |
Pseudomonas putida | N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | - |
Arthrobacter sp. | N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | - |
Thermus sp. | N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | - |
Flavobacterium sp. | N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | - |
Pseudomonas sp. NS671 | N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | - |
Arthrobacter sp. DSM 3747 | N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | - |
Flavobacterium sp. AJ-3912 | N-carbamoyl glycine | - |
? | |
| L-5-(1-methylpropyl)hydantoin + H2O | - |
Geobacillus stearothermophilus | N-carbamoyl-L-isoleucine | - |
? | |
| L-5-(2-methylthioethyl)hydantoin + H2O | - |
Geobacillus stearothermophilus | N-carbamoyl-L-methionine | - |
? | |
| L-5-benzylhydantoin + H2O | - |
Geobacillus stearothermophilus | N-carbamoyl-L-phenylalanine | - |
? | |
| L-5-isobutylhydantoin + H2O | - |
Geobacillus stearothermophilus | N-carbamoyl-L-leucine | - |
? | |
| L-5-isobutylhydantoin + H2O | - |
Geobacillus stearothermophilus NS1122A | N-carbamoyl-L-leucine | - |
? | |
| L-5-methylhydantoin + H2O | - |
Geobacillus stearothermophilus | N-carbamoyl-L-alanine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | - |
Bos taurus |
| tetramer | - |
Pseudomonas fluorescens |
| tetramer | 4 * 55000, SDS-PAGE | Geobacillus stearothermophilus |
| tetramer | 4 * 55000-62000 | Arthrobacter sp. |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
L-5-methylhydantoin as substrate | Geobacillus stearothermophilus |
| 60 | 70 | resting cells | Geobacillus stearothermophilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Pseudomonas fluorescens |
| 8 | - |
resting cells | Geobacillus stearothermophilus |
| 9.5 | - |
- |
Pseudomonas putida |
| 9.5 | - |
L-5-methylhydantoin as substrate | Geobacillus stearothermophilus |
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