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Literature summary for 3.5.2.2 extracted from
- Purification, characterization and inhibition of dihydropyrimidinase from rat liver (1994), Eur. J. Biochem., 219, 393-399.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2,6-dipicolinic acid | - |
Bos taurus |  |
| 2,6-dipicolinic acid | - |
Rattus norvegicus | |
| 8-Hydroxyquinoline-5-sulfonic acid | - |
Bos taurus | |
| 8-Hydroxyquinoline-5-sulfonic acid | - |
Rattus norvegicus | |
| N-Carbamoyl-beta-alanine | product inhibition | Rattus norvegicus | |
| N-carbamoyl-beta-aminoisobutyric acid | product inhibition | Rattus norvegicus | |
| Zn2+ | irreversible inhibition | Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.017 | - |
5,6-Dihydrothymine | pH 8.2 | Rattus norvegicus | |
| 0.017 | - |
5-bromo-5,6-dihydrouracil | pH 8.2 | Rattus norvegicus | |
| 0.019 | - |
5,6-dihydrouracil | pH 7.0 | Rattus norvegicus | |
| 0.02 | - |
5,6-dihydrouracil | pH 8.2 | Rattus norvegicus | |
| 0.025 | - |
5-bromo-5,6-dihydrouracil | pH 7.0 | Rattus norvegicus | |
| 0.026 | - |
5,6-Dihydrothymine | pH 7.0 | Rattus norvegicus | |
| 1.19 | - |
N-carbamoyl-beta-aminoisobutyric acid | pH 7.0 | Rattus norvegicus | |
| 1.59 | - |
N-carbamoyl-beta-aminoisobutyric acid | pH 8.2 | Rattus norvegicus | |
| 1.92 | - |
N-Carbamoyl-beta-alanine | pH 7.0 | Rattus norvegicus | |
| 2.26 | - |
N-Carbamoyl-beta-alanine | pH 8.2 | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Rattus norvegicus | 5829 | - |
| cytosol | - |
Bos taurus | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | essential, metalloenzyme | Rattus norvegicus | |
| Zn2+ | essential, metalloenzyme | Bos taurus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 54000 | - |
4 * 54000, SDS-PAGE | Rattus norvegicus |
| 215000 | - |
gel filtration | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,6-dihydropyrimidine + H2O | Rattus norvegicus | - |
beta-ureido acid | - |
r | |
| 5,6-dihydropyrimidine + H2O | Bos taurus | - |
beta-ureido acid | - |
r | |
| 5,6-dihydrothymine + H2O | Rattus norvegicus | - |
? | - |
r | |
| 5,6-dihydrothymine + H2O | Bos taurus | - |
? | - |
r | |
| 5,6-dihydrouracil + H2O | Bos taurus | - |
3-ureidopropionate | - |
r | |
| hydantoin + H2O | Homo sapiens | - |
N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | Rattus norvegicus | - |
N-carbamoyl glycine | - |
r | |
| hydantoin + H2O | Bos taurus | - |
N-carbamoyl glycine | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
calf | - |
| Bos taurus | - |
bovine | - |
| Homo sapiens | - |
human | - |
| Rattus norvegicus | - |
rat | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
- |
Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| ascites | - |
Rattus norvegicus | - |
| brain | - |
Rattus norvegicus | - |
| heart | - |
Rattus norvegicus | - |
| kidney | - |
Rattus norvegicus | - |
| kidney | - |
Bos taurus | - |
| liver | - |
Rattus norvegicus | - |
| liver | - |
Bos taurus | - |
| lung | - |
Rattus norvegicus | - |
| small intestine | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 14.8 | - |
- |
Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, purified enzyme, 20% decrease in specific activity observed within 2 months | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-methylhydantoin + H2O | poor substrate | Rattus norvegicus | 3-methylhydantoic acid | - |
? | |
| 5,6-dihydropyrimidine + H2O | - |
Rattus norvegicus | beta-ureido acid | - |
r | |
| 5,6-dihydropyrimidine + H2O | - |
Bos taurus | beta-ureido acid | - |
r | |
| 5,6-dihydrothymine + H2O | - |
Rattus norvegicus | ? | - |
r | |
| 5,6-dihydrothymine + H2O | - |
Bos taurus | ? | - |
r | |
| 5,6-dihydrouracil + H2O | - |
Rattus norvegicus | 3-ureidopropionate | - |
r | |
| 5,6-dihydrouracil + H2O | - |
Bos taurus | 3-ureidopropionate | - |
r | |
| 5-bromo-5,6-dihydrouracil + H2O | - |
Rattus norvegicus | ? | - |
? | |
| 5-bromo-5,6-dihydrouracil + H2O | - |
Bos taurus | ? | - |
? | |
| 5-fluoro-5,6-dihydrouracil + H2O | - |
Rattus norvegicus | ? | - |
? | |
| carbamoyl beta-aminoisobutyric acid + H2O | - |
Rattus norvegicus | ? | - |
? | |
| carbamoyl beta-aminoisobutyric acid + H2O | - |
Bos taurus | ? | - |
? | |
| D-5-methylhydantoin + H2O | - |
Bos taurus | N-carbamoyl-D-alanine | - |
? | |
| D-5-methylhydantoin + H2O | L-5-methylhydantoin is no substrate | Rattus norvegicus | N-carbamoyl-D-alanine | - |
? | |
| dihydropyrimidine + H2O | - |
Rattus norvegicus | N-carbamoyl amino acid | - |
r | |
| dihydropyrimidine + H2O | - |
Bos taurus | N-carbamoyl amino acid | - |
r | |
| DL-5-isopropylhydantoin + H2O | only D-isomer hydrolyzed | Rattus norvegicus | N-carbamoyl-D-valine | - |
? | |
| glutarimide + H2O | - |
Rattus norvegicus | ? | - |
? | |
| glutarimide + H2O | - |
Bos taurus | ? | - |
? | |
| hydantoin + H2O | - |
Homo sapiens | N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | - |
Rattus norvegicus | N-carbamoyl glycine | - |
r | |
| hydantoin + H2O | - |
Bos taurus | N-carbamoyl glycine | - |
r | |
| additional information | - |
Bos taurus | ? | - |
? | |
| additional information | succinimide, dihydro-L-orotic-2-acid, dihydrouridine and dihydrothymidine are no substrates | Rattus norvegicus | ? | - |
? | |
| N-carbamoyl beta-alanine + H2O | - |
Rattus norvegicus | 5,6-dihydrouracil | - |
r | |
| N-carbamoyl beta-alanine + H2O | - |
Bos taurus | 5,6-dihydrouracil | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | - |
Bos taurus |
| tetramer | 4 * 54000, SDS-PAGE | Rattus norvegicus |
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