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Literature summary for 3.5.2.2 extracted from

  • Brooks, K.P.; Jones, E.A.; Kim, B.D.; Sander, E.G.
    Bovine liver dihydropyrimidine amidohydrolase: Purification, properties, and characterization as a zinc metalloenzyme (1983), Arch. Biochem. Biophys., 226, 469-483.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
2,6-dipicolinic acid
-
Bos taurus
4-nitrobenzene sulfonamide
-
Bos taurus
8-Hydroxyquinoline-5-sulfonic acid
-
Bos taurus
o-phenanthroline
-
Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.008
-
5,6-dihydrouracil
-
Bos taurus
0.015
-
Dihydrothymine
-
Bos taurus
0.015
-
beta-ureidopropionate 0.1 mM Bos taurus
0.018
-
5-iododihydrouracil
-
Bos taurus
0.02
-
5-bromodihydrouracil
-
Bos taurus
0.028
-
beta-Ureidoisobutyrate 0.1 mM Bos taurus
0.069
-
beta-Ureidoisobutyrate 0.1-3.25 mM Bos taurus
0.079
-
beta-ureidopropionate 0.1-5 mM Bos taurus
50
-
hydantoin
-
Bos taurus
62.5
-
5-methylhydantoin
-
Bos taurus

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ essential, metalloenzyme Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56500
-
4 * 56500, SDS-PAGE Bos taurus
226000
-
gel filtration Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5,6-dihydropyrimidine + H2O Bos taurus
-
beta-ureido acid
-
r
5,6-dihydrothymine + H2O Bos taurus
-
?
-
r
5,6-dihydrouracil + H2O Bos taurus
-
3-ureidopropionate
-
?
hydantoin + H2O Bos taurus
-
N-carbamoyl glycine
-
?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
beef
-

Purification (Commentary)

Purification (Comment) Organism
-
Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
Bos taurus
-
liver
-
Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
10.1
-
-
Bos taurus
10.4
-
-
Bos taurus

Storage Stability

Storage Stability Organism
4°C, stable in dilute Tris buffer pH 7.5 for several days with no loss of activity, purified enzyme loses up to 50% activity after 6 months Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,6-dihydropyrimidine + H2O
-
Bos taurus beta-ureido acid
-
r
5,6-dihydrothymine + H2O
-
Bos taurus ?
-
r
5,6-dihydrouracil + H2O
-
Bos taurus 3-ureidopropionate
-
?
5-bromo-5,6-dihydrouracil + H2O
-
Bos taurus ?
-
r
5-iodo-5,6-dihydrouracil + H2O
-
Bos taurus ?
-
?
beta-ureidoisobutyrate
-
Bos taurus dihydrothymine + H2O
-
r
D-5-methylhydantoin + H2O
-
Bos taurus N-carbamoyl-D-alanine
-
?
DL-5-methylhydantoin
-
Bos taurus ?
-
?
hydantoin + H2O
-
Bos taurus N-carbamoyl glycine
-
?
additional information L-dihydroorotic acid is no substrate Bos taurus ?
-
?
N-carbamoyl beta-alanine + H2O
-
Bos taurus 5,6-dihydrouracil
-
r
succinimide + H2O
-
Bos taurus ?
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 56500, SDS-PAGE Bos taurus