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Literature summary for 3.5.2.2 extracted from

  • Morin, A.; Hummel, W.; Kula, M.R.
    Production of hydantoinase from Pseudomonas fluorescens strain DSM 84 (1986), Appl. Microbiol. Biotechnol., 25, 91-96.
No PubMed abstract available
Search for more literature for 3.5.2.2

Inhibitors

Inhibitors Comment Organism Structure
D-glucose
-
 Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5,6-dihydrouracil + H2O Pseudomonas fluorescens
-
 3-ureidopropionate
-
 ?
5,6-dihydrouracil + H2O Pseudomonas putida
-
 3-ureidopropionate
-
 ?
5,6-dihydrouracil + H2O Agrobacterium rhizogenes
-
 3-ureidopropionate
-
 ?
5,6-dihydrouracil + H2O Agrobacterium tumefaciens
-
 3-ureidopropionate
-
 ?
5,6-dihydrouracil + H2O Agrobacterium rubi
-
 3-ureidopropionate
-
 ?
5,6-dihydrouracil + H2O Agrobacterium tumefaciens DSM 30147
-
 3-ureidopropionate
-
 ?
5,6-dihydrouracil + H2O Agrobacterium rubi DSM 30149
-
 3-ureidopropionate
-
 ?
5,6-dihydrouracil + H2O Agrobacterium rhizogenes DSM 30148
-
 3-ureidopropionate
-
 ?
5,6-dihydrouracil + H2O Agrobacterium tumefaciens DSM 30150
-
 3-ureidopropionate
-
 ?
hydantoin + H2O Pseudomonas fluorescens
-
 N-carbamoyl glycine
-
 ?
hydantoin + H2O Pseudomonas putida
-
 N-carbamoyl glycine
-
 ?
hydantoin + H2O Agrobacterium rhizogenes
-
 N-carbamoyl glycine
-
 ?
hydantoin + H2O Agrobacterium tumefaciens
-
 N-carbamoyl glycine
-
 ?
hydantoin + H2O Agrobacterium sp.
-
 N-carbamoyl glycine
-
 ?
hydantoin + H2O Agrobacterium rubi
-
 N-carbamoyl glycine
-
 ?
hydantoin + H2O Agrobacterium tumefaciens DSM 30147
-
 N-carbamoyl glycine
-
 ?
hydantoin + H2O Agrobacterium rubi DSM 30149
-
 N-carbamoyl glycine
-
 ?
hydantoin + H2O Agrobacterium rhizogenes DSM 30148
-
 N-carbamoyl glycine
-
 ?
hydantoin + H2O Agrobacterium tumefaciens DSM 30150
-
 N-carbamoyl glycine
-
 ?
hydantoin + H2O Agrobacterium sp. DSM 30201
-
 N-carbamoyl glycine
-
 ?

Organism

Organism UniProt Comment Textmining
Agrobacterium rhizogenes
-
 and strains DSM 30199, DSM 30200
-
Agrobacterium rhizogenes DSM 30148
-
 and strains DSM 30199, DSM 30200
-
Agrobacterium rubi
-
 and strains DSM 30149, DSM 30155
-
Agrobacterium rubi DSM 30149
-
 and strains DSM 30149, DSM 30155
-
Agrobacterium sp.
-
 and strain DSM 30203
-
Agrobacterium sp. DSM 30201
-
 and strain DSM 30203
-
Agrobacterium tumefaciens
-
 and strains DSM 30199, NRRL B-11291
-
Agrobacterium tumefaciens
-
 and strains DSM 30150, DSM 30205, DSM 30206, DSM 30207
-
Agrobacterium tumefaciens DSM 30147
-
 and strains DSM 30199, NRRL B-11291
-
Agrobacterium tumefaciens DSM 30150
-
 and strains DSM 30150, DSM 30205, DSM 30206, DSM 30207
-
Pseudomonas fluorescens
-
 strain DSM 84
-
Pseudomonas putida
-
 formerly classified as P. striata
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas fluorescens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.005
-
 strain 30207, isopropylhydantoin as substrate Agrobacterium tumefaciens
0.006
-
 strain 30201, isopropylhydantoin as substrate Agrobacterium sp.
0.007
-
 strain 30205, isopropylhydantoin as substrate Agrobacterium tumefaciens
0.009
-
 strain 30199, isopropylhydantoin as substrate Agrobacterium tumefaciens
0.01
-
 strain 50106, isopropylhydantoin as substrate Pseudomonas fluorescens
0.011
-
 strain 2005, isopropylhydantoin as substrate Pseudomonas fluorescens
0.013
-
 strain 30150, isopropylhydantoin as substrate Agrobacterium tumefaciens
0.014
-
 strain 50106, incubated with hydantoin, isopropylhydantoin as substrate Pseudomonas fluorescens
0.014
-
 strain 50108, isopropylhydantoin as substrate Pseudomonas fluorescens
0.015
-
 strain 50090, incubated with hydantoin, isopropylhydantoin as substrate Pseudomonas fluorescens
0.016
-
 strain 11291, isopropylhydantoin as substrate Agrobacterium tumefaciens
0.016
-
 strain 50090, isopropylhydantoin as substrate Pseudomonas fluorescens
0.019
-
 strain 50154, isopropylhydantoin as substrate Pseudomonas fluorescens
0.021
-
 strain 30204, isopropylhydantoin as substrate Agrobacterium tumefaciens
0.022
-
 strain 2005, isopropylhydantoin as substrate Pseudomonas fluorescens
0.023
-
 strain 30147, isopropylhydantoin as substrate Agrobacterium tumefaciens
0.033
-
 strain 50108, isopropylhydantoin as substrate Pseudomonas fluorescens
0.033
-
 strain 30201, dihydrouracil as substrate Agrobacterium sp.
0.034
-
 strain 291, isopropylhydantoin as substrate Pseudomonas putida
0.038
-
 strain 84, isopropylhydantoin as substrate Pseudomonas fluorescens
0.049
-
 strain 549, isopropylhydantoin as substrate Pseudomonas putida
0.051
-
 strain 291, incubated with hydantoin, isopropylhydantoin as substrate Pseudomonas putida
0.059
-
 strain 11291, dihydrouracil as substrate Agrobacterium tumefaciens
0.094
-
 strain 11291, incubated with hydantoin Agrobacterium tumefaciens
0.112
-
 strain 30205, dihydrouracil as substrate Agrobacterium tumefaciens
0.127
-
 strain 549, incubated with hydantoin, isopropylhydantoin as substrate Pseudomonas putida
0.128
-
 strain 30150, dihydrouracil as substrate Agrobacterium tumefaciens
0.15
-
 strain 2005, dihydrouracil as substrate Pseudomonas fluorescens
0.185
-
 strain 84, incubated with hydantoin, isopropylhydantoin as substrate Pseudomonas fluorescens
0.19
-
 strain 50154, dihydrouracil as substrate Pseudomonas fluorescens
0.193
-
 strain 30204, dihydrouracil as substrate Agrobacterium tumefaciens
0.194
-
 strain 50090, dihydrouracil as substrate Pseudomonas fluorescens
0.212
-
 strain 30147, dihydrouracil as substrate Agrobacterium tumefaciens
0.299
-
 strain 30155, dihydrouracil as substrate Agrobacterium rubi
0.31
-
 strain 50106, dihydrouracil as substrate Pseudomonas fluorescens
0.317
-
 strain 30208, dihydrouracil as substrate Agrobacterium tumefaciens
0.425
-
 strain 30207, dihydrouracil as substrate Agrobacterium tumefaciens
0.5
-
 strain 291, dihydrouracil as substrate Pseudomonas putida
0.53
-
 strain 50108, dihydrouracil as substrate Pseudomonas fluorescens
0.562
-
 strain 30206, dihydrouracil as substrate Agrobacterium tumefaciens
0.62
-
 strain 2005, incubated with hydantoin, dihydrouracil as substrate Pseudomonas fluorescens
0.73
-
 strain 549, incubated with hydantoin, dihydrouracil as substrate Pseudomonas putida
0.78
-
 dihydrouracil as substrate Pseudomonas fluorescens
0.81
-
 strain 50154, incubated with hydantoin, dihydrouracil as substrate Pseudomonas fluorescens
0.98
-
 strain 50108, incubated with hydantoin, dihydrouracil as substrate Pseudomonas fluorescens
1.11
-
 strain 50106, incubated with hydantoin, dihydrouracil as substrate Pseudomonas fluorescens
1.14
-
 strain 30155, dihydrouracil as substrate Agrobacterium rubi
1.2
-
 strain 549, dihydrouracil as substrate Pseudomonas putida
1.4
-
 strain 50090, incubated with hydantoin, dihydrouracil as substrate Pseudomonas fluorescens
1.9
-
 strain 549, incubated with hydantoin, dihydrouracil as substrate Pseudomonas putida
2.25
-
 incubated with hydantoin Pseudomonas fluorescens

Storage Stability

Storage Stability Organism
4°C, potassium phosphate buffer at pH 8 permits best enzyme recovery and assured stability of enzyme for more than 14 days Pseudomonas fluorescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,6-dihydrouracil + H2O
-
 Pseudomonas fluorescens 3-ureidopropionate
-
 ?
5,6-dihydrouracil + H2O
-
 Pseudomonas putida 3-ureidopropionate
-
 ?
5,6-dihydrouracil + H2O
-
 Agrobacterium rhizogenes 3-ureidopropionate
-
 ?
5,6-dihydrouracil + H2O
-
 Agrobacterium tumefaciens 3-ureidopropionate
-
 ?
5,6-dihydrouracil + H2O
-
 Agrobacterium sp. 3-ureidopropionate
-
 ?
5,6-dihydrouracil + H2O
-
 Agrobacterium rubi 3-ureidopropionate
-
 ?
5,6-dihydrouracil + H2O
-
 Agrobacterium tumefaciens DSM 30147 3-ureidopropionate
-
 ?
5,6-dihydrouracil + H2O
-
 Agrobacterium rubi DSM 30149 3-ureidopropionate
-
 ?
5,6-dihydrouracil + H2O
-
 Agrobacterium rhizogenes DSM 30148 3-ureidopropionate
-
 ?
5,6-dihydrouracil + H2O
-
 Agrobacterium tumefaciens DSM 30150 3-ureidopropionate
-
 ?
5,6-dihydrouracil + H2O
-
 Agrobacterium sp. DSM 30201 3-ureidopropionate
-
 ?
5-(p-hydroxybenzyl)hydantoin + H2O
-
 Pseudomonas fluorescens ?
-
 ?
DL-5-(2-methylthioethyl)-hydantoin + H2O
-
 Pseudomonas putida N-carbamoyl-D-methionine
-
 ?
DL-5-(2-methylthioethyl)-hydantoin + H2O
-
 Agrobacterium tumefaciens N-carbamoyl-D-methionine
-
 ?
DL-5-(2-methylthioethyl)-hydantoin + H2O
-
 Agrobacterium sp. N-carbamoyl-D-methionine
-
 ?
DL-5-(2-methylthioethyl)-hydantoin + H2O
-
 Pseudomonas fluorescens N-carbamoyl-D-methionine steric configuration D ?
DL-5-(2-methylthioethyl)-hydantoin + H2O
-
 Agrobacterium tumefaciens DSM 30147 N-carbamoyl-D-methionine
-
 ?
DL-5-(2-methylthioethyl)-hydantoin + H2O
-
 Agrobacterium tumefaciens DSM 30150 N-carbamoyl-D-methionine
-
 ?
DL-5-(2-methylthioethyl)-hydantoin + H2O
-
 Agrobacterium sp. DSM 30201 N-carbamoyl-D-methionine
-
 ?
DL-5-isopropylhydantoin + H2O
-
 Pseudomonas fluorescens N-carbamoyl-D-valine
-
 ?
DL-5-isopropylhydantoin + H2O
-
 Agrobacterium tumefaciens N-carbamoyl-D-valine
-
 ?
DL-5-isopropylhydantoin + H2O
-
 Agrobacterium sp. N-carbamoyl-D-valine
-
 ?
DL-5-isopropylhydantoin + H2O
-
 Agrobacterium tumefaciens DSM 30147 N-carbamoyl-D-valine
-
 ?
DL-5-isopropylhydantoin + H2O
-
 Agrobacterium tumefaciens DSM 30150 N-carbamoyl-D-valine
-
 ?
DL-5-isopropylhydantoin + H2O
-
 Agrobacterium sp. DSM 30201 N-carbamoyl-D-valine
-
 ?
hydantoin + H2O
-
 Pseudomonas fluorescens N-carbamoyl glycine
-
 ?
hydantoin + H2O
-
 Pseudomonas putida N-carbamoyl glycine
-
 ?
hydantoin + H2O
-
 Agrobacterium rhizogenes N-carbamoyl glycine
-
 ?
hydantoin + H2O
-
 Agrobacterium tumefaciens N-carbamoyl glycine
-
 ?
hydantoin + H2O
-
 Agrobacterium sp. N-carbamoyl glycine
-
 ?
hydantoin + H2O
-
 Agrobacterium rubi N-carbamoyl glycine
-
 ?
hydantoin + H2O
-
 Agrobacterium tumefaciens DSM 30147 N-carbamoyl glycine
-
 ?
hydantoin + H2O
-
 Agrobacterium rubi DSM 30149 N-carbamoyl glycine
-
 ?
hydantoin + H2O
-
 Agrobacterium rhizogenes DSM 30148 N-carbamoyl glycine
-
 ?
hydantoin + H2O
-
 Agrobacterium tumefaciens DSM 30150 N-carbamoyl glycine
-
 ?
hydantoin + H2O
-
 Agrobacterium sp. DSM 30201 N-carbamoyl glycine
-
 ?

Synonyms

Synonyms Comment Organism
microbial hydantoinase
-
 Pseudomonas fluorescens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
27
-
-
 Pseudomonas fluorescens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 stable at temperatures up to, starts to lose significant activity at 50°C, remaining activity at 55°C less than 25%, heat treatment in presence of substrate stabilizes enzyme at temperatures in excess of 40°C Pseudomonas fluorescens
51
-
 more than 40% activity lost after 10 min Pseudomonas fluorescens

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.1 7.2 more stable in slightly alkaline buffers Pseudomonas fluorescens