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Literature summary for 3.5.2.2 extracted from
- Pyrimidine catabolism: individual characterization of the three sequential enzymes with a new assay (1984), Biochemistry, 23, 2533-2539.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.011 | - |
5,6-dihydrouracil | - |
Rattus norvegicus |  |
| 117 | - |
5,6-dihydrouracil | - |
Bos taurus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Rattus norvegicus | 5829 | - |
| cytosol | - |
Bos taurus | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 140000 | - |
- |
Bos taurus |
| 226000 | - |
- |
Bos taurus |
| 226000 | - |
gel filtration | Rattus norvegicus |
| 266000 | - |
- |
Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,6-dihydrouracil + H2O | Cavia porcellus | - |
3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | Mus musculus | - |
3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | Escherichia coli | - |
3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | Bos taurus | - |
3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | Oryctolagus cuniculus | - |
3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | Euglena gracilis | - |
3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | Canis lupus familiaris | - |
3-ureidopropionate | - |
? | |
| hydantoin + H2O | Cavia porcellus | - |
N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | Mus musculus | - |
N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | Escherichia coli | - |
N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | Rattus norvegicus | - |
N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | Bos taurus | - |
N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | Oryctolagus cuniculus | - |
N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | Euglena gracilis | - |
N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | Canis lupus familiaris | - |
N-carbamoyl glycine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
calf | - |
| Canis lupus familiaris | - |
dog | - |
| Cavia porcellus | - |
guinea-pig | - |
| Escherichia coli | - |
- |
- |
| Euglena gracilis | - |
- |
- |
| Mus musculus | - |
mouse | - |
| Oryctolagus cuniculus | - |
rabbit | - |
| Rattus norvegicus | - |
rat | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| hepatoma cell | - |
Rattus norvegicus | - |
| kidney | - |
Cavia porcellus | - |
| kidney | - |
Mus musculus | - |
| kidney | - |
Rattus norvegicus | - |
| kidney | - |
Bos taurus | - |
| kidney | - |
Oryctolagus cuniculus | - |
| kidney | - |
Canis lupus familiaris | - |
| liver | - |
Cavia porcellus | - |
| liver | - |
Mus musculus | - |
| liver | - |
Rattus norvegicus | - |
| liver | - |
Bos taurus | - |
| liver | - |
Oryctolagus cuniculus | - |
| liver | - |
Canis lupus familiaris | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, no loss of activity for several months | Rattus norvegicus |
| 4°C, less than 20% activity lost after 2-3 days | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,6-dihydrouracil + H2O | - |
Cavia porcellus | 3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | - |
Mus musculus | 3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | - |
Escherichia coli | 3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | - |
Rattus norvegicus | 3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | - |
Bos taurus | 3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | - |
Oryctolagus cuniculus | 3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | - |
Euglena gracilis | 3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | - |
Canis lupus familiaris | 3-ureidopropionate | - |
? | |
| 5-fluoro-5,6-dihydrouracil + H2O | less effective substrate | Rattus norvegicus | ? | - |
? | |
| hydantoin + H2O | - |
Cavia porcellus | N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | - |
Mus musculus | N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | - |
Escherichia coli | N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | - |
Rattus norvegicus | N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | - |
Bos taurus | N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | - |
Oryctolagus cuniculus | N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | - |
Euglena gracilis | N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | - |
Canis lupus familiaris | N-carbamoyl glycine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | - |
Rattus norvegicus |
| tetramer | - |
Bos taurus |
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