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Literature summary for 3.5.2.17 extracted from

  • Yamauchi, K.; Kasai, K.
    Sequential molecular events of functional trade-offs in 5-hydroxyisourate hydrolase before and after gene duplication led to the evolution of transthyretin during chordate diversification (2018), J. Mol. Evol., 86, 457-469 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Rosetta 2(DE3)pLysS Oncorhynchus mykiss
expression in Escherichia coli Rosetta 2(DE3)pLysS Branchiostoma japonicum

Protein Variants

Protein Variants Comment Organism
R54E/Y119T amino acid substitutions, R54E/Y119T, at the active sites of HIUHase, exert weak [125I]-3,3',5-triiodo-l-thyronine([125I]T3) binding activity with a concomitant loss of 5-hydroxyisourate hydrolysis activity Oncorhynchus mykiss
R54E/Y119T amino acid substitutions, R54E/Y119T, at the active sites of HIUHase, exert weak [125I]-3,3',5-triiodo-l-thyronine([125I]T3) binding activity with a concomitant loss of 5-hydroxyisourate hydrolysis activity Branchiostoma japonicum

Inhibitors

Inhibitors Comment Organism Structure
additional information addition of 3His to the N-terminal end weakens 5-hydroxyisourate hydrolysis activity, whereas it enhanced T3-binding activity of R54E/Y119T mutsant enzyme Branchiostoma japonicum
additional information addition of 3His to the N-terminal end weakens 5-hydroxyisourate hydrolysis activity, whereas it enhanced T3-binding activity of R54E/Y119T mutsant enzyme Oncorhynchus mykiss

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5-hydroxyisourate + H2O Oncorhynchus mykiss
-
5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate
-
?
5-hydroxyisourate + H2O Branchiostoma japonicum
-
5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate
-
?

Organism

Organism UniProt Comment Textmining
Branchiostoma japonicum K9MS40
-
-
Oncorhynchus mykiss K9MS40
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Oncorhynchus mykiss
-
Branchiostoma japonicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-hydroxyisourate + H2O
-
Oncorhynchus mykiss 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate
-
?
5-hydroxyisourate + H2O
-
Branchiostoma japonicum 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate
-
?

Synonyms

Synonyms Comment Organism
5-hydroxyisourate hydrolase
-
Oncorhynchus mykiss
5-hydroxyisourate hydrolase
-
Branchiostoma japonicum
HIUHase
-
Oncorhynchus mykiss
HIUHase
-
Branchiostoma japonicum

General Information

General Information Comment Organism
evolution sequential molecular events of functional trade-offs in 5-hydroxyisourate hydrolase before and after gene duplication led to the evolution of transthyretin during chordate diversification Oncorhynchus mykiss
evolution sequential molecular events of functional trade-offs in 5-hydroxyisourate hydrolase before and after gene duplication led to the evolution of transthyretin during chordate diversification Branchiostoma japonicum
metabolism the enzyme is involved in urate metabolism Oncorhynchus mykiss
metabolism the enzyme is involved in urate metabolism Branchiostoma japonicum