Literature summary for 3.5.2.17 extracted from

Zanotti, G.; Cendron, L.; Ramazzina, I.; Folli, C.; Percudani, R.; Berni, R.
Structure of zebra fish HIUase: insights into evolution of an enzyme to a hormone transporter (2006), J. Mol. Biol., 363, 1-9.

Search for more literature for 3.5.2.17

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21 (DE3) cells	Danio rerio

Crystallization (Commentary)

Crystallization (Comment)	Organism
at 4°C by the sitting-drop, vapour diffusion method, using 0.1 M HEPES (pH 7.5), 20% (w/v) PEG 10000	Danio rerio

Protein Variants

Protein Variants	Comment	Organism
D50N	shows 50% activity of the wild type enzyme	Danio rerio

Organism

Organism	UniProt	Comment	Textmining
Danio rerio	Q06S87	-	-

Purification (Commentary)

Purification (Comment)	Organism
Q-Sepharose column chromatography	Danio rerio

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
5-hydroxyisourate + H2O	-	Danio rerio	5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate	-	?

Subunits

Subunits	Comment	Organism
homotetramer	x-ray crystallography	Danio rerio

Synonyms

Synonyms	Comment	Organism
5-hydroxyisourate hydrolase	-	Danio rerio
HIUase	-	Danio rerio

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Release 2023.1 (January 2023)