Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta BL21 cells | Salmonella enterica subsp. enterica serovar Dublin |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapour diffusion method in 0.1 M sodium cacodylate (pH 6.0), 0.2 M MgCl2, 20% (w/v) polyethylene glycol 3350 at 22°C | Salmonella enterica subsp. enterica serovar Dublin |
Protein Variants | Comment | Organism |
---|---|---|
H6A | inactive | Salmonella enterica subsp. enterica serovar Dublin |
H95A | 90% reduced activity | Salmonella enterica subsp. enterica serovar Dublin |
Y108F | 90% reduced activity | Salmonella enterica subsp. enterica serovar Dublin |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
13000 | - |
4 * 13000, SDS-PAGE | Salmonella enterica subsp. enterica serovar Dublin |
52000 | - |
SDS-PAGE | Salmonella enterica subsp. enterica serovar Dublin |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica subsp. enterica serovar Dublin | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Superose-12 gel filtration and HiTrap SP column chromatography | Salmonella enterica subsp. enterica serovar Dublin |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-hydroxyisourate + H2O | - |
Salmonella enterica subsp. enterica serovar Dublin | 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 13000, SDS-PAGE | Salmonella enterica subsp. enterica serovar Dublin |
Synonyms | Comment | Organism |
---|---|---|
5-hydroxyisourate hydrolase | - |
Salmonella enterica subsp. enterica serovar Dublin |
TLP | - |
Salmonella enterica subsp. enterica serovar Dublin |
transthyretin-like protein | - |
Salmonella enterica subsp. enterica serovar Dublin |