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Literature summary for 3.5.2.17 extracted from
- Cloning and expression of the gene for soybean hydroxyisourate hydrolase. Localization and implications for function and mechanism (2002), Plant Physiol., 130, 2061-2068.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloned and expressed in Escherichia coli | Glycine max |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Glycine max |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E199A | site-directed mutagenesis, mutant devoid of detectable catalytic activity | Glycine max |
| E408A | site-directed mutagenesis, mutant devoid of detectable catalytic activity | Glycine max |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.02 | - |
5-hydroxyisourate | pH 7.5, 37°C, recombinant enzyme lacking the signal peptide | Glycine max |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| peroxisome | - |
Glycine max | 5777 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 63730 | - |
calculated from cDNA sequence | Glycine max |
| 68000 | - |
SDS-PAGE, gel filtration | Glycine max |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-hydroxyisourate + H2O | Glycine max | - |
5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Glycine max | - |
- |
- |
| Glycine max | Q8S3J3 | soybean, cv Williams | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Glycine max |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-hydroxyisourate + H2O | - |
Glycine max | 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HIUHase | - |
Glycine max |
| hydroxyisourate hydrolase | - |
Glycine max |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 24 | - |
5-hydroxyisourate | pH 7.5, 37°C, recombinant enzyme lacking the signal peptide | Glycine max | |
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