Cloned (Comment) | Organism |
---|---|
His-tagged enzyme expessed in Escherichia coli BL21 (DE3)pLysS | Glycine max |
Protein Variants | Comment | Organism |
---|---|---|
E199A | activity is reduced 400fold relative to wild-type | Glycine max |
E408A | mutant with no activity | Glycine max |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.026 | - |
5,6-diaminouracil | pH 7.5, 30°C | Glycine max | |
11 | - |
5-hydroxyisourate | pH 7.5, 30°C | Glycine max |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-hydroxyisourate + H2O | Glycine max | - |
5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate | - |
? | |
5-hydroxyisourate + H2O | Glycine max | ureide pathway | 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycine max | - |
- |
- |
Glycine max | - |
soybean | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Glycine max |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,6-diaminouracil + H2O | slow alternative substrate | Glycine max | ? | - |
? | |
5-hydroxyisourate + H2O | - |
Glycine max | 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate | - |
? | |
5-hydroxyisourate + H2O | ureide pathway | Glycine max | 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate | - |
? | |
additional information | alloxan is no substrate | Glycine max | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HIUHase | - |
Glycine max |
hydroxyisourate hydrolase | - |
Glycine max |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
5-hydroxyisourate | pH 7.5, 30°C | Glycine max | |
0.21 | - |
5,6-diaminouracil | pH 7.5, 30°C | Glycine max |