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Literature summary for 3.5.2.16 extracted from
- Gene cloning, expression, and substrate specificity of an imidase from the strain Pseudomonas putida YZ-26. (2007), Curr. Microbiol., 55, 61-64.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpressed in Escherichia coli | Pseudomonas putida |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 29.2 | - |
succinimide | - |
Pseudomonas putida |  |
| 39.1 | - |
Maleimide | - |
Pseudomonas putida | |
| 53.5 | - |
hydantoin | - |
Pseudomonas putida | |
| 74.3 | - |
dihydrouracil | - |
Pseudomonas putida | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36000 | - |
x * 36000, (His)6-tagged enzyme, SDS-PAGE | Pseudomonas putida |
| 141000 | - |
gel filtration | Pseudomonas putida |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | Q4JG22 | - |
- |
| Pseudomonas putida YZ-26 | Q4JG22 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Pseudomonas putida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dihydrouracil + H2O | - |
Pseudomonas putida | N-carbamoyl-beta-alanine | - |
? | |
| dihydrouracil + H2O | - |
Pseudomonas putida YZ-26 | N-carbamoyl-beta-alanine | - |
? | |
| hydantoin + H2O | - |
Pseudomonas putida | hydantoic acid monoamide | - |
? | |
| hydantoin + H2O | - |
Pseudomonas putida YZ-26 | hydantoic acid monoamide | - |
? | |
| maleimide + H2O | - |
Pseudomonas putida | maleic acid monoamide | - |
? | |
| maleimide + H2O | - |
Pseudomonas putida YZ-26 | maleic acid monoamide | - |
? | |
| additional information | no activity with DL-5-phenylhydantoin and DL-p-hydroxyphenylhydantoin | Pseudomonas putida | ? | - |
? | |
| additional information | no activity with DL-5-phenylhydantoin and DL-p-hydroxyphenylhydantoin | Pseudomonas putida YZ-26 | ? | - |
? | |
| succinimide + H2O | - |
Pseudomonas putida | succinamic acid monoamide | - |
? | |
| succinimide + H2O | - |
Pseudomonas putida YZ-26 | succinamic acid monoamide | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 33712.6, calculated from sequence | Pseudomonas putida |
| ? | x * 36000, (His)6-tagged enzyme, SDS-PAGE | Pseudomonas putida |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.107 | - |
hydantoin | - |
Pseudomonas putida | |
| 2.94 | - |
hydantoin | - |
Pseudomonas putida | |
| 9.14 | - |
dihydrouracil | - |
Pseudomonas putida | |
| 479 | - |
Maleimide | - |
Pseudomonas putida | |
| 1494 | - |
succinimide | - |
Pseudomonas putida | |
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