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Literature summary for 3.5.2.16 extracted from
- Rat liver imidase (1993), J. Biol. Chem., 268, 10870-10874.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| N-Carbamoyl-beta-alanine | - |
Rattus norvegicus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2 | - |
Dihydrothymine | pH 9.5, 30°C | Rattus norvegicus | |
| 0.5 | - |
6-methyldihydrouracil | pH 9.5, 30°C | Rattus norvegicus | |
| 2 | - |
N-Carbamoyl-beta-alanine | pH 5.4, 30°C | Rattus norvegicus | |
| 2.2 | - |
dihydrouracil | pH 9.5, 30°C | Rattus norvegicus | |
| 3.9 | - |
Phthalimide | pH 6.5, 30°C | Rattus norvegicus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | only the two physiological substrates dihydrouracil and dihydrothymine are hydrolyzed at a greater rate in the presence of 5 mM MgSO4 | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 53000 | - |
4 * 53000, SDS-PAGE | Rattus norvegicus |
| 230000 | - |
gel filtration | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 6-methyldihydrouracil + H2O | Rattus norvegicus | - |
3-ureidobutanoic acid | - |
? | |
| dihydrothymine + H2O | Rattus norvegicus | - |
? | - |
? | |
| dihydrouracil + H2O | Rattus norvegicus | cyclization is catalyzed with much lower activity and affinity | N-carbamoyl-beta-alanine | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
thermophilic enzyme | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE-cellulose, polyethylene glycol precipitation, DEAE-Sephacel, isoelectric focusing, hydroxyapatite, DEAE-Sephacel, gel filtration | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 27 | - |
- |
Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3,4-pyridinedicarboximide + H2O | - |
Rattus norvegicus | 4-carbamoyl-alpha-picolinic acid | - |
? | |
| 6-methyldihydrouracil + H2O | - |
Rattus norvegicus | 3-ureidobutanoic acid | - |
? | |
| dihydrothymine + H2O | - |
Rattus norvegicus | ? | - |
? | |
| dihydrouracil + H2O | cyclization is catalyzed with much lower activity and affinity | Rattus norvegicus | N-carbamoyl-beta-alanine | - |
r | |
| phthalimide + H2O | - |
Rattus norvegicus | 2-(aminocarbonyl)benzoic acid | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 53000, SDS-PAGE | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 9.5 | optimal pH correlates with pKa of the substrates amide proton | Rattus norvegicus |
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