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Literature summary for 3.5.2.10 extracted from
- Crystallization and preliminary crystallographic analysis of creatininase from Pseudomonas putida (2002), Acta Crystallogr. Sect. D, 58, 1356-1358.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Pseudomonas putida |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| sitting drop vapour diffusion method with 22% ethanol, 20% PEG 8000, and 0.1 M MOPS pH 7.5 | Pseudomonas putida |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28399 | - |
6 * 28399, calculated from amino acid sequence | Pseudomonas putida |
| 28400 | - |
6 * 28400, MALDI-TOF mass spectrometry | Pseudomonas putida |
| 170400 | - |
SDS-PAGE | Pseudomonas putida |
| 173000 | - |
dynamic light scattering | Pseudomonas putida |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| creatinine + H2O | - |
Pseudomonas putida | creatine | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homohexamer | 6 * 28399, calculated from amino acid sequence | Pseudomonas putida |
| homohexamer | 6 * 28400, MALDI-TOF mass spectrometry | Pseudomonas putida |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CrnA | - |
Pseudomonas putida |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | the enzyme tends to precipitate at pH values of under 6.0 and over 9.0 corresponding to the stability range of the enzyme | Pseudomonas putida |
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