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Literature summary for 3.5.2.10 extracted from
- Creatinine amidohydrolase (creatininase) from Pseudomonas putida. Purification and some properties (1979), J. Biochem., 86, 1109-1117.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | - |
Pseudomonas putida |  |
| Cu2+ | - |
Pseudomonas putida | |
| EDTA | - |
Pseudomonas putida | |
| ethoxyformic anhydride | - |
Pseudomonas putida | |
| Fe2+ | - |
Pseudomonas putida | |
| heavy metal ions | - |
Pseudomonas putida | |
| Mg2+ | - |
Pseudomonas putida | |
| additional information | - |
Pseudomonas putida | |
| N-bromosuccinimide | - |
Pseudomonas putida | |
| o-phenanthroline | - |
Pseudomonas putida | |
| PCMB | - |
Pseudomonas putida | |
| Photooxidation | - |
Pseudomonas putida |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 26 | - |
creatinine | - |
Pseudomonas putida | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | activates | Pseudomonas putida | |
| Fe2+ | activates | Pseudomonas putida | |
| Mg2+ | activates | Pseudomonas putida | |
| Mn2+ | activates | Pseudomonas putida | |
| Ni2+ | activates | Pseudomonas putida | |
| Zn2+ | one gatom Zn2+ per mol of subunit monomer | Pseudomonas putida | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 23000 | - |
8 * 23000, SDS-PAGE | Pseudomonas putida |
| 175000 | - |
ultracentrifugal analysis | Pseudomonas putida |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| creatinine + H2O | Pseudomonas putida | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | - |
var. naraensis, strain C-83 | - |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| unstable to photooxidation | Pseudomonas putida |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | 3.5% glucose | Pseudomonas putida |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purified by column chromarographies on sarcosine-HM-Sepharose and DEAE-cellulose, and gel filtration on Sephadex G-200 | Pseudomonas putida |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 488 | - |
- |
Pseudomonas putida |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 6 months | Pseudomonas putida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| creatinine + H2O | - |
Pseudomonas putida | creatine | - |
? | |
| creatinine + H2O | - |
Pseudomonas putida | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| octamer | 8 * 23000, SDS-PAGE | Pseudomonas putida |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
30 min at pH 7.1, apoenzyme | Pseudomonas putida |
| 70 | - |
30 min at pH 7.1, native enzyme | Pseudomonas putida |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | - |
Pseudomonas putida |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 12 | for 3 h at 30°C | Pseudomonas putida |
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