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Literature summary for 3.5.2.1 extracted from
- Barbiturase, a novel zinc-containing amidohydrolase involved in oxidative pyrimidine metabolism (2002), J. Biol. Chem., 277, 7051-7058.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Rhodococcus erythropolis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2,2'-dipyridyl | 2 mM, 30% inhibition | Rhodococcus erythropolis |  |
| 5,5'-dithio-bis-(2-nitrobenzoic acid) | 2 mM, 50% inhibition | Rhodococcus erythropolis | |
| 8-hydroxyquinoline | 2 mM, 60% inhibition | Rhodococcus erythropolis | |
| alloxan | competitive | Rhodococcus erythropolis | |
| Cd2+ | 2 mM, 60% inhibition | Rhodococcus erythropolis | |
| Co2+ | 2 mM, 50% inhibition | Rhodococcus erythropolis | |
| Cu2+ | 2 mM, complete inhibition | Rhodococcus erythropolis | |
| cyanuric acid | competitive | Rhodococcus erythropolis | |
| dihydro-L-orotate | competitive | Rhodococcus erythropolis | |
| diisopropyl phosphofluoridate | 2 mM, 90% inhibition | Rhodococcus erythropolis | |
| EDTA | 2 mM, 30% inhibition | Rhodococcus erythropolis | |
| Hg2+ | 2 mM, complete inhibition | Rhodococcus erythropolis | |
| N-bromosuccinimide | 2 mM, 60% inhibition | Rhodococcus erythropolis | |
| N-ethylmaleimide | 2 mM, complete inhibition | Rhodococcus erythropolis | |
| Ni2+ | 2 mM, 80% inhibition | Rhodococcus erythropolis | |
| o-phenanthroline | 2 mM, 80% inhibition | Rhodococcus erythropolis | |
| p-chloromercuribenzoate | 2 mM, complete inhibition | Rhodococcus erythropolis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1 | - |
Barbiturate | pH 8.0, 30°C | Rhodococcus erythropolis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | 4.4 mol per mol of enzyme | Rhodococcus erythropolis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
4 * 45000, SDS-PAGE | Rhodococcus erythropolis |
| 172000 | - |
gel filtration | Rhodococcus erythropolis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhodococcus erythropolis | Q8RSQ2 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rhodococcus erythropolis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 10% (v/v) ethylene glykol, stable up to 3 months | Rhodococcus erythropolis |
| 4°C, cell-free extract, loss of 70% of activity during 2 weeks | Rhodococcus erythropolis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| barbiturate + H2O | strict specificity | Rhodococcus erythropolis | ureidomalonic acid | - |
? | |
| ureidomalonic acid | - |
Rhodococcus erythropolis | barbiturate + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | 4 * 45000, SDS-PAGE | Rhodococcus erythropolis |
| More | partial amino acid sequence analysis | Rhodococcus erythropolis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
30 min, complete loss of activity | Rhodococcus erythropolis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
loss of activity above | Rhodococcus erythropolis |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.42 | - |
cyanuric acid | pH 8.0, 30°C | Rhodococcus erythropolis | |
| 1.7 | - |
alloxan | pH 8.0, 30°C | Rhodococcus erythropolis | |
| 4.5 | - |
dihydro-L-orotate | pH 8.0, 30°C | Rhodococcus erythropolis | |
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Release 2023.1 (January 2023)
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